2002年2月
Cloning and expression of an alpha-L-arabinofuranosidase gene (stxIV) from Streptomyces thermoviolaceus OPC-520, and characterization of the enzyme
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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- 巻
- 66
- 号
- 2
- 開始ページ
- 434
- 終了ページ
- 438
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1271/bbb.66.434
- 出版者・発行元
- TAYLOR & FRANCIS LTD
The gene encoding alpha-L-arabinofuranosidase (STX-IV), located upstream of the previously reported stxI gene, was cloned and sequenced. The gene is divergently transcribed from the stxI gene, and the two genes are separated by 661 nucleotides. The stxI V gene consists of a 1,092-bp open reading frame encoding 363 amino acids. The deduced amino acid sequence of the gene showed that STX-IV was an enzyme consisting of only a catalytic domain, and that the enzyme had significant similarity with mu-L-arabinofuranosidases belonging to family 62 of glycosyl hydrolases. The stxI V gene was expressed in Escherichia coli, and the recombinant protein was purified to homogeneity. Arabinoxylan and oat spelt xylan were good substrates for STX-IV, however, the enzyme showed a low activity with p-nitrophenyl mu-L-arabinofuranoside. The optimum pH and temperature were 5.0 and 60degreesC, respectively.
- リンク情報
- ID情報
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- DOI : 10.1271/bbb.66.434
- ISSN : 0916-8451
- eISSN : 1347-6947
- PubMed ID : 11999423
- Web of Science ID : WOS:000174150400032