2013年10月
表面開始ATRP法によりLCST型温度応答性高分子を修飾したガラス基板の調製とHeLa細胞の温度刺激はく離
近畿大学工学部研究報告
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- 巻
- 47
- 号
- 47
- 開始ページ
- 7
- 終了ページ
- 12
- 記述言語
- 日本語
- 掲載種別
- 出版者・発行元
- 近畿大学工学部
[Abstract] For high-throughput cell-diagnosis and non-invasive target cell recovery by using microarrays, the surface-modified glass substrates with a controlled poly(N-isopropylacrylamide) [poly(NiPAAm)] length were prepared by Activators ReGenerated by Electron Transfer surface initiated atom transfer radical polymerization(ARGET SI-ATRP). Polymer chain length was increased by the increase of the polymerization time (2h, 6h). From the Scanning probe microscopy(SPM) measurements, poly(NiPAAm) grafted glass observed polymer brush. From the contact angle(θ)measurements to water at 25 ℃, the θ values of poly(NiPAAm) grafted glass decreased from θ=56°(the untreated glass) to θ=43. The contact angle(θ)measurements to water at 40℃, the θ values of poly(NiPAAm) grafted glass increased from θ=43°to θ=52. HeLa cells attached and proliferated on g-glass at 37℃, above the lower critical solution temperature of poly(NiPAAm). After the cultivation of HeLa cells, the thermal stimuli-exfoliation were also examined at 25℃ for 1 h. The rations of number of thermal stimuli exfoliation cells to the total number of attached on g-glass was 95%, while little thermal stimuli-exfoliation was obtained in the case of untreated glass.[注記]NOUSOU, Tatsuki
- リンク情報
- ID情報
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- ISSN : 0386-491X
- CiNii Articles ID : 120005735865
- CiNii Books ID : AN00063799