YOSHIDA Toyokazu

J-GLOBAL         Last updated: Jul 2, 2019 at 02:42
 
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Name
YOSHIDA Toyokazu
Affiliation
Gifu University
Section
Faculty of Engineering
Degree
(BLANK)(Kyoto University)

Research Areas

 
 

Academic & Professional Experience

 
 
   
 
Gifu University Faculty of Engineering, Department of Biomolecular Science
 
1989
 - 
1996
Kinki University, Research Assistant
 
1996
 - 
1997
Kinki University, Research Associate
 

Education

 
 
 - 
1989
Graduate School, Division of Agriculture, Kyoto University
 
 
 - 
1987
Faculty of Agriculture, Kyoto University
 

Published Papers

 
Yoshida Toyokazu(Gifu University),Nagasawa Toru(Gifu University)
62 21-26   Mar 2003
Purification and characterization of epsilon-poly-L-lysine-degrading enzyme from epsilon-poly-L-lysine-torelant Chryseobacerium sp. OJ7
Kito Mitsuaki(Gifu University),Takimoto Rika(Gifu University),Onji Yuichi(Chisso Corporation),Yoshida Toyokazu(Gifu University),Nagasawa Toru(Gifu University)
96 92-94   May 2003
Purification, characterization and gene cloning of thermostable O-acetyl-L-serine sulfhydrylase forming beta-cyano-L-alanine
Omura Hironori(Gifu University, Ikeda Food Research Corporation),Kuroda Masako(Ikeda Food Research Corporation),Kobayashi Michihiko(Kyoto University),Shimizu Sakayu(Kyoto University),Yoshida Toyokazu(Gifu University),Nagasawa Toru(Gifu University)
95 470-475   May 2003
Uchida Akira(Gifu University),Yoshida Toyokazu(Gifu University),Ogawa Mina(Gifu University),Nagasawa Toru(Gifu University)
62 337-341   Jun 2003
Uchida Akira(Gifu University),Ogawa Mina(Gifu University),Yoshida Toyokazu(Gifu University),Nagasawa Toru(Gifu University)
180 81-87   Oct 2003

Misc

 
Koichi Mitsukura, Tatsuya Kuramoto, Toyokazu Yoshida, Norihiro Kimoto, Hiroaki Yamamoto, Toru Nagasawa
Applied Microbiology and Biotechnology   97 8079-8086
A NADPH-dependent (S)-imine reductase (SIR) was purified to be homogeneous from the cell-free extract of Streptomyces sp. GF3546. SIR appeared to be a homodimer protein with subunits of 30.5 kDa based on SDS-polyacrylamide gel electrophoresis and ...
Koichi Mitsukura, Mai Suzuki, Kazuhiro Tada, Toyokazu Yoshida, Toru Nagasawa
Organic and Biomolecular Chemistry   8 4533-4535
Streptomyces sp. GF3587 and 3546 were found to be imine-reducing strains with high R- and S-selectivity by screening using 2-methyl-1-pyrroline (2-MPN). Their whole-cell catalysts produced 91 mM R-2-methylpyrrolidine (R-2-MP) with 99.2%e.e. and 27...
Koichi Mitsukura, Hiromu Sakamoto, Haruka Kubo, Toyokazu Yoshida, Toru Nagasawa
Journal of Bioscience and Bioengineering   109 550-553
To efficiently produce 1,3-adamantanediol (1,3-ad(OH)2) from 1-adamantanol (1-adOH), our stocks of culture strains and soil microorganisms were surveyed for hydroxylation activity towards 1-adOH. Among them, the soil actinomycete SA8 showing the h...
Toyokazu Yoshida, Yuki Sada, Toru Nagasawa
Applied Microbiology and Biotechnology   86 1165-1170   Apr 2010
Alkane-assimilating microorganisms were isolated from enrichment cultures using n-octane, n-dodecane, nhexadecane, or pristane (2,6,10,14- tetramethylpentadecane) as a sole carbon source to find microbial catalysts oxidizing methyl groups of 2,6-d...
Masaki Torimura, Hideto Yoshida, Kenji Kano, Tokuji Ikeda, Toyokazu Yoshida, Toru Nagasawa
Journal of Molecular Catalysis - B Enzymatic   8 265-273
Pseudomonas fluorescens TN5 catalyzes the hydroxylation of nicotinic acid (NA) into 6-hydroxynicotinic acid (6HNA), an important compound as a starting material for the synthesis of a new type of pesticides. Under aerobic conditions, however, 6HNA...

Books etc

 
epsilon-Poly-L-lysine
Yoshida Toyokazu(Gifu University),Hiraki Jyun(Chisso Corporation),NagasawaToru(Gifu University)
Feb 2003   
Biological Kobe-Schmitt carboxylation
Yoshida Toyokazu(Gifu University),Nagasawa Toru(Gifu University)
Jul 2007   

Research Grants & Projects

 
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(C))
Project Year: 2011 - 2013    Investigator(s): Toyokazu YOSHIDA
The existence of 2,4-dihydroxybenzoate decarboxylase had been suggested in anaerobic microorganisms. However, microorganisms had not yet be isolated. Therefore, the decarboxylase had not be characterized. In the present study, microorganisms sh...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(C))
Project Year: 2006 - 2007    Investigator(s): Toyokazu YOSHIDA
Various enzymes catalyzing oxidative or nonoxidative decarboxylation reaction are involved in aerobic or anaerobic degradation of aromatic carboxylic acids in microorganisms. Recently, we found and characterized several kinds of novel decarboxylas...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(C))
Project Year: 2004 - 2005    Investigator(s): Toyokazu YOSHIDA
Various enzymes catalyzing oxidative or nonoxidative decarboxylation reaction are involved in aerobic or anaerobic degradation of aromatic carboxylic acids in microorganisms. Recently, we found and characterized two kinds of novel decarboxylase, c...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(C))
Project Year: 2002 - 2003    Investigator(s): Toyokazu YOSHIDA
Generally, decarboxylases can not catalyze the reverse carboxylation reaction. However, we found novel decarboxylases efficiently catalyzing nonoxidative decarboxyation. In the metabolism of phenol in the absence of oxygen, 4-hydroxybenzoate decar...
Molecular conversion by novel microbial C1 fixation system