2010年5月
Regioselective carboxylation of catechol by 3,4-dihydroxybenzoate decarboxylase of Enterobacter cloacae P
BIOTECHNOLOGY LETTERS
- ,
- ,
- ,
- 巻
- 32
- 号
- 5
- 開始ページ
- 701
- 終了ページ
- 705
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s10529-010-0210-3
- 出版者・発行元
- SPRINGER
3,4-Dihydroxybenzoate decarboxylase in Enterobacter cloacae P241 was induced by adding 3,4-dihydroxybenzoic acid, 3-hydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid or 4-acetamidobenzoic acid to the culture medium. After stabilizing the enzyme activity by adding 5 mM dithiothreitol and 20 mM Na(2)S(2)O(3) to a cell-free extract, catechol at 50 mM was carboxylated in the presence of 3 M KHCO(3) to 3,4-dihydroxybenzoic acid with a molar conversion ratio of 28% after 14 h at 30A degrees C.
- リンク情報
- ID情報
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- DOI : 10.1007/s10529-010-0210-3
- ISSN : 0141-5492
- Web of Science ID : WOS:000276509300015