MISC

1991年8月

A MOUSE DNA-REPAIR ENZYME (APEX NUCLEASE) HAVING EXONUCLEASE AND APURINIC APYRIMIDINIC ENDONUCLEASE ACTIVITIES - PURIFICATION AND CHARACTERIZATION

BIOCHIMICA ET BIOPHYSICA ACTA
  • S SEKI
  • ,
  • S IKEDA
  • ,
  • S WATANABE
  • ,
  • M HATSUSHIKA
  • ,
  • K TSUTSUI
  • ,
  • K AKIYAMA
  • ,
  • B ZHANG

1079
1
開始ページ
57
終了ページ
64
記述言語
英語
掲載種別
出版者・発行元
ELSEVIER SCIENCE BV

A mouse repair enzyme having priming activity on bleomycin-damaged DNA for DNA polymerase was purified to apparent homogeneity and characterized. The enzyme extracted from permeabilized mouse ascites sarcoma (SR-C3H / He) cells with 0.2 M potassium phosphate buffer (pH 7.5) was purified by successive chromatographies on phosphocellulose, DEAE-cellulose, phosphocellulose (a second time), Sephadex G-100, single-stranded DNA cellulose and hydroxyapatite. The purified enzyme has an M(r) of 34000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Enzymatical studies indicated that it is a multifunctional enzyme having exonuclease, apurinic/apyrimidinic endonuclease and phosphatase activities, similar to Escherichia coli exonuclease III. This enzyme is tentatively designated as APEX nuclease for apurinic/apyrimidinic endonuclease and exonuclease activities. The amino acid composition, amino-terminal amino acid sequence and an internal amino acid sequence of APEX nuclease are determined.


リンク情報
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1991GD60600009&DestApp=WOS_CPL

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