Aug, 1991
A MOUSE DNA-REPAIR ENZYME (APEX NUCLEASE) HAVING EXONUCLEASE AND APURINIC APYRIMIDINIC ENDONUCLEASE ACTIVITIES - PURIFICATION AND CHARACTERIZATION
BIOCHIMICA ET BIOPHYSICA ACTA
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- Volume
- 1079
- Number
- 1
- First page
- 57
- Last page
- 64
- Language
- English
- Publishing type
- Publisher
- ELSEVIER SCIENCE BV
A mouse repair enzyme having priming activity on bleomycin-damaged DNA for DNA polymerase was purified to apparent homogeneity and characterized. The enzyme extracted from permeabilized mouse ascites sarcoma (SR-C3H / He) cells with 0.2 M potassium phosphate buffer (pH 7.5) was purified by successive chromatographies on phosphocellulose, DEAE-cellulose, phosphocellulose (a second time), Sephadex G-100, single-stranded DNA cellulose and hydroxyapatite. The purified enzyme has an M(r) of 34000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Enzymatical studies indicated that it is a multifunctional enzyme having exonuclease, apurinic/apyrimidinic endonuclease and phosphatase activities, similar to Escherichia coli exonuclease III. This enzyme is tentatively designated as APEX nuclease for apurinic/apyrimidinic endonuclease and exonuclease activities. The amino acid composition, amino-terminal amino acid sequence and an internal amino acid sequence of APEX nuclease are determined.
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- ID information
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- ISSN : 0006-3002
- Web of Science ID : WOS:A1991GD60600009