MISC

1996年11月

Identification and characterization of a mitochondrial endonuclease from yeast, Schizosaccharomyces pombe

BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL
  • S Ikeda
  • ,
  • N Maeda
  • ,
  • T Ohshima
  • ,
  • N Takata

40
5
開始ページ
1017
終了ページ
1024
記述言語
英語
掲載種別
出版者・発行元
ACADEMIC PRESS AUST

Schizosaccharomyces pombe mitochondria were isolated from the cells treated with Novozyme 234, and purified in a Percoll gradient. A zymographic assay in a SDS-polyacrylamide gel containing single-stranded DNA revealed that an endonuclease of 32 kDa is associated with the mitochondria. The endonuclease was extracted from the mitochondria with 0.5 M KCI and was partially purified. The 32-kDa enzyme degraded both DNA and RNA at a weak alkaline pH, but preferred single-stranded DNA. The enzyme required Mg2+ or Mn2+, but not Ca2+ or Zn2+ for activity, and was inhibited by 50% with a 150 mM salt solution. Nicks generated by the enzyme could be resealed with T4 DNA ligase, indicating that the enzyme produces 5'-P and 3'-OH ends.

Web of Science ® 被引用回数 : 11

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Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1996VX09700019&DestApp=WOS_CPL

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