MISC

1997年6月

Action of mitochondrial endonuclease G on DNA damaged by L-ascorbic acid, peplomycin, and cis-diamminedichloroplatinum (II)

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • S Ikeda
  • ,
  • K Ozaki

235
2
開始ページ
291
終了ページ
294
記述言語
英語
掲載種別
DOI
10.1006/bbrc.1997.6786
出版者・発行元
ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS

Mitochondrial DNA (mtDNA) suffers extensive damage from the environment, however, the enzymes involved in the repair of mtDNA are still unknown. Here, we partially purified mitochondrial endonuclease G (Endo G) from the bovine heart, and examined the action of Endo G; on damaged DNA, Treatment of DNA with L-ascorbic acid or peplomycin, that introduces single-strand breaks through active oxygen radicals, greatly enhanced the susceptibility to nucleolytic attacks from Endo Gr, The enzyme cleaved at or near sites where single-strand breaks were present in the opposite strand, Cisplatin-mediated DNA damage, which causes intrastrand crosslinks between adjacent guanine residues, also facilitated Endo G digestion, indicating that the enzyme can recognize local distortions in the duplex DNA introduced by adducts, These nucleolytic properties of Endo G in vitro suggest its possible involvement in the maintenance of mtDNA by eliminating defective genomes from the multicopy pool. (C) 1997 Academic Press.

Web of Science ® 被引用回数 : 42

リンク情報
DOI
https://doi.org/10.1006/bbrc.1997.6786
CiNii Articles
http://ci.nii.ac.jp/naid/80009727948
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/9199184
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1997XF76700005&DestApp=WOS_CPL

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