MISC

2001年5月

Isolation and characterization of the Schizosaccharomyces pombe cDNA encoding the mitochondrial endonuclease

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
  • S Ikeda
  • ,
  • N Kawasaki

1519
1-2
開始ページ
111
終了ページ
116
記述言語
英語
掲載種別
DOI
10.1016/S0167-4781(01)00206-8
出版者・発行元
ELSEVIER SCIENCE BV

We isolated the cDNA of the fission yeast mitochondrial endonuclease SpNUC1, which consists of 322 amino acids and has a significant homology with the budding yeast NUC1 and mammalian endonuclease G. Comparison of the cDNA sequence with the genomic sequence showed that the gene consists of three exons and two introns and spans 1.31 kb. The enzyme localization in mitochondria was demonstrated by expressing the SpNUC1-green fluorescent protein fusion in the yeast. The endonuclease was activated by truncation of the aminoterminal region of the protein, indicating that the enzyme is encoded as an inactive precursor. The active enzyme degraded single-stranded DNA and RNA, the activity being dependent on Mg2+ (Mn2+). (C) 2001 Elsevier Science B.V. All rights reserved.

Web of Science ® 被引用回数 : 13

リンク情報
DOI
https://doi.org/10.1016/S0167-4781(01)00206-8
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000169415500014&DestApp=WOS_CPL

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