2006年12月
Identification of a functional 2-keto-myo-inositol dehydratase gene of Sinorhizobium fredii USDA191 required for myo-inositol utilization
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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- 巻
- 70
- 号
- 12
- 開始ページ
- 2957
- 終了ページ
- 2964
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1271/bbb.60362
- 出版者・発行元
- TAYLOR & FRANCIS LTD
Sinorhizobium fredii USDA191 is a Gram-negative bacterium capable of forming nitrogen-fixing nodules on soybean roots. The USDA191 idhA gene encoding myo-inositol dehydrogenase, an enzyme necessary for myo-inositol utilization, is known to be involved in competitive nodulation and nitrogen fixation. In Bacillus subtilis, myo-inositol dehydrogenase catalyzes the first step of the myo-inositol catabolic pathway. Recently WE was identified as the gene encoding 2-keto-myo-inositol dehydratase, which catalyzes the second step in the pathway. Here we report the presence of 2-keto-myo-inositol dehydratase activity in free-living USDA191 cells cultured in a medium containing myo-inositol. An WE ortholog was cloned from USDA191. USDA191 WE was expressed in Escherichia coli as a His(6)-tag fusion and purified to exhibit 2-keto-myo-inositol dehydratase activity. Inactivation of USDA191 WE led to defective myo-inositol utilization. USDA191 iolE partially complemented a B. subtilis WE deficient mutant. These results suggest that S. fredii USDA191 utilizes a myoinositol catabolic pathway, analogous to that of B. subtilis, involving at least idhA and WE.
- リンク情報
- ID情報
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- DOI : 10.1271/bbb.60362
- ISSN : 0916-8451
- eISSN : 1347-6947
- CiNii Articles ID : 10018523881
- Web of Science ID : WOS:000243219300019