MISC

査読有り
2009年6月

Regulation of the Bacillus subtilis Divergent yetL and yetM Genes by a Transcriptional Repressor, YetL, in Response to Flavonoids

JOURNAL OF BACTERIOLOGY
  • Kazutake Hirooka
  • ,
  • Yusuke Danjo
  • ,
  • Yuki Hanano
  • ,
  • Satoshi Kunikane
  • ,
  • Hiroshi Matsuoka
  • ,
  • Shigeo Tojo
  • ,
  • Yasutaro Fujita

191
11
開始ページ
3685
終了ページ
3697
記述言語
英語
掲載種別
DOI
10.1128/JB.00202-09
出版者・発行元
AMER SOC MICROBIOLOGY

DNA microarray analysis revealed that transcription of the Bacillus subtilis yetM gene encoding a putative flavin adenine dinucleotide-dependent monooxygenase was triggered by certain flavonoids during culture and was derepressed by disruption of the yetL gene in the opposite orientation situated immediately upstream of yetM, which encodes a putative MarR family transcriptional regulator. In vitro analyses, including DNase I footprinting and gel retardation analysis, indicated that YetL binds specifically to corresponding single sites in the divergent yetL and yetM promoter regions, with higher affinity to the yetM region; the former region overlaps the Shine-Dalgarno sequence of yetL, and the latter region contains a perfect 18-bp palindromic sequence (TAGTTAGGCGCCTAACTA). In vitro gel retardation and in vivo lacZ fusion analyses indicated that some flavonoids (kaempferol, apigenin, and luteolin) effectively inhibit YetL binding to the yetM cis sequence, but quercetin, galangin, and chrysin do not inhibit this binding, implying that the 4-hydroxyl group on the B-ring of the flavone structure is indispensable for this inhibition and that the coexistence of the 3-hydroxyl groups on the B- and C-rings does not allow antagonism of YetL.

リンク情報
DOI
https://doi.org/10.1128/JB.00202-09
CiNii Articles
http://ci.nii.ac.jp/naid/80020318300
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/19329649
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000266041300028&DestApp=WOS_CPL
ID情報
  • DOI : 10.1128/JB.00202-09
  • ISSN : 0021-9193
  • CiNii Articles ID : 80020318300
  • PubMed ID : 19329649
  • Web of Science ID : WOS:000266041300028

エクスポート
BibTeX RIS