1995年9月
SPECIFIC RECOGNITION OF THE BACILLUS-SUBTILIS GNT CIS-ACTING CATABOLITE-RESPONSIVE ELEMENT BY A PROTEIN COMPLEX FORMED BETWEEN CCPA AND SERYL-PHOSPHORYLATED HPR
MOLECULAR MICROBIOLOGY
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回数 : 196
- ,
- ,
- ,
- 巻
- 17
- 号
- 5
- 開始ページ
- 953
- 終了ページ
- 960
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1111/j.1365-2958.1995.mmi_17050953.x
- 出版者・発行元
- BLACKWELL SCIENCE LTD
Catabolite repression of various Bacillus subtilis catabolic operons which carry a cis-acting catabolite-responsive element (CRE), such as the gnt operon, is mediated by CcpA, a protein belonging to the GalR-Lacl family of bacterial transcriptional repressors/activators, and the seryl-phosphorylated form of HPr, a phosphocarrier protein of the phosphoenolpyruvate:sugar phosphotransferase system. Footprinting experiments revealed that the purified CcpA protein interacted with P-ser-HPr to cause specific protection of the gnt CRE against DNase I digestion. The specific recognition of the gnf CRE was confirmed by the results of footprinting experiments using mutant gnt CREs carrying one of the following base substitutions within the CRE consensus sequence: G to T at position +149 or C to T at position +154 (+1 is the gnt transcription initiation nucleotide). The two mutant CREs causing a partial relief from catabolite repression were not protected by the CcpA/P-ser-HPr complex in footprinting experiments. Based on these and previous findings, we propose a molecular mechanism underlying catabolite repression in B. subtilis mediated by CcpA and P-ser-HPr.
- リンク情報
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- DOI
- https://doi.org/10.1111/j.1365-2958.1995.mmi_17050953.x
- CiNii Articles
- http://ci.nii.ac.jp/naid/80008613211
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/8596444
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1995RZ99400015&DestApp=WOS_CPL
- URL
- http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.1995.mmi_17050953.x/abstract
- ID情報
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- DOI : 10.1111/j.1365-2958.1995.mmi_17050953.x
- ISSN : 0950-382X
- CiNii Articles ID : 80008613211
- PubMed ID : 8596444
- Web of Science ID : WOS:A1995RZ99400015