論文

査読有り 最終著者 責任著者 国際誌
2016年3月

The Effect of Exercise on the Early Stages of Mesenchymal Stromal Cell-Induced Cartilage Repair in a Rat Osteochondral Defect Model

PLOS ONE
  • Shoki Yamaguchi
  • ,
  • Tomoki Aoyama
  • ,
  • Akira Ito
  • ,
  • Momoko Nagai
  • ,
  • Hirotaka Iijima
  • ,
  • Junichi Tajino
  • ,
  • Xiangkai Zhang
  • ,
  • Wataru Kiyan
  • ,
  • Hiroshi Kuroki

11
3
開始ページ
e0151580
終了ページ
e0151580
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0151580
出版者・発行元
PUBLIC LIBRARY SCIENCE

The repair of articular cartilage is challenging owing to the restriction in the ability of articular cartilage to repair itself. Therefore, cell supplementation therapy is possible cartilage repair method. However, few studies have verified the efficacy and safety of cell supplementation therapy. The current study assessed the effect of exercise on early the phase of cartilage repair following cell supplementation utilizing mesenchymal stromal cell (MSC) intra-articular injection. An osteochondral defect was created on the femoral grooves bilaterally of Wistar rats. Mesenchymal stromal cells that were obtained from male Wistar rats were cultured in monolayer. After 4 weeks, MSCs were injected into the right knee joint and the rats were randomized into an exercise or no-exercise intervention group. The femurs were divided as follows: C group (no exercise without MSC injection); E group (exercise without MSC injection); M group (no exercise with MSC injection); and ME group (exercise with MSC injection). At 2, 4, and 8 weeks after the injection, the femurs were sectioned and histologically graded using the Wakitani cartilage repair scoring system. At 2 weeks after the injection, the total histological scores of the M and ME groups improved significantly compared with those of the C group. Four weeks after the injection, the scores of both the M and ME groups improved significantly. Additionally, the scores in the ME group showed a significant improvement compared to those in the M group. The improvement in the scores of the E, M, and ME groups at 8 weeks were not significantly different. The findings indicate that exercise may enhance cartilage repair after an MSC intra-articular injection. This study highlights the importance of exercise following cell transplantation therapy.

リンク情報
DOI
https://doi.org/10.1371/journal.pone.0151580
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26968036
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4788414
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000371989200065&DestApp=WOS_CPL
ID情報
  • DOI : 10.1371/journal.pone.0151580
  • ISSN : 1932-6203
  • PubMed ID : 26968036
  • PubMed Central 記事ID : PMC4788414
  • Web of Science ID : WOS:000371989200065

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