The latissimus dorsi (LD) can be divided into the upper, middle, and lower parts, but the effective stretching positions for each part are unknown. In this study, we aimed to investigate effective trunk positions for stretching of the LD. A total of 14 healthy males participated in this study. The following seven trunk positions were chosen as the LD stretching positions; upright of the trunk (Baseline), flexion of the trunk (Flex), contralateral bending of the trunk (LB), contralateral rotation of the trunk (Rot), flexion and contralateral bending of the trunk (Flex + LB), flexion and contralateral rotation of the trunk (Flex + Rot), and contralateral bending and contralateral rotation of the trunk (LB + Rot). Maximal elevation of the upper limb was passively added to all positions. The shear elastic modulus, used as the index of muscle elongation, was measured at the four parts (upper, middle, lower, distal parts) of the LD. The shear elastic moduli showed obviously high values in Rot and LB + Rot at the upper, middle, and distal parts, and also in LB, Rot, and LB + Rot at the lower part. These findings suggest that contralateral trunk rotation, or a combination of contralateral trunk bending and rotation are effective trunk positions for stretching all parts of the LD. Contralateral trunk bending was also effective for stretching the lower part of the LD.