論文

査読有り
2012年11月

Discovery of a new source of resistance to Fusarium oxysporum, cause of Fusarium wilt in Allium fistulosum, located on chromosome 2 of Allium cepa Aggregatum group

GENOME
  • Hoa Q. Vu
  • ,
  • Magdi A. El-Sayed
  • ,
  • Shin-Ichi Ito
  • ,
  • Naoki Yamauchi
  • ,
  • Masayoshi Shigyo

55
11
開始ページ
797
終了ページ
807
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1139/g2012-065
出版者・発行元
CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS

This study was carried out to evaluate the antifungal effect of Allium cepa Aggregatum group (shallot) metabolites on Fusarium oxysporum and to determine the shallot chromosome(s) related to Fusarium wilt resistance using a complete set of eight Allium fistulosum - shallot monosomic addition lines. The antifungal effects of hexane, butanol, and water extraction fractions from bulbs of shallot on 35 isolates of F. oxysporum were examined using the disc diffusion method. Only hexane and butanol fractions showed high antifungal activity. Shallot showed no symptom of disease after inoculation with F. oxysporum f. sp. cepae. The phenolic content of the roots and the saponin content of root exudates of inoculated shallot increased to much higher levels than those of the control at 3 days after inoculation. Application of freeze-dried shallot root exudates to seeds of A. fistulosum soaked in a spore suspension of F. oxysporum resulted in protection of seedlings against infection. Among eight monosomic addition lines and A. fistulosum, FF + 2A showed the highest resistance to Fusarium wilt. This monosomic addition line also showed a specific saponin band derived from shallot on the thin layer chromatography profile of saponins in the eight monosomic addition lines. The chromosome 2A of shallot might possess some of the genes related to Fusarium wilt resistance.

リンク情報
DOI
https://doi.org/10.1139/g2012-065
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000311897800006&DestApp=WOS_CPL
ID情報
  • DOI : 10.1139/g2012-065
  • ISSN : 0831-2796
  • Web of Science ID : WOS:000311897800006

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