2006年8月
A unique monoclonal antibody against human plasma fibronectin, which recognizes an epitope on the surface of a subpopulation of polymorphonuclear neutrophils
HYBRIDOMA
- ,
- 巻
- 25
- 号
- 4
- 開始ページ
- 202
- 終了ページ
- 208
- 記述言語
- 英語
- 掲載種別
- 出版者・発行元
- MARY ANN LIEBERT INC
A new monoclonal antibody (MAb) specific to plasma fibronectin, named MO, which was produced by immunizing a mouse with fragments of fibronectin isolated from human plasma, was found to bind to the surface of a subpopulation of peripheral blood polymorphonuclear neutrophils (PMNs) in the presence of physiological concentration of soluble fibronectin. MAb MO had no reactivity with other blood cells such as lymphocytes, monocytes, or platelets. The percentage of the MAb MO-reactive subpopulation of PMNs from healthy donors varied from 0 to 60%. Ten previously established MAbs against fibronectin tested in this study together with MAb MO had no reactivity with the surface of PMNs at all, when tested simultaneously with MAb MO. These results indicate that MAb MO can be a unique and useful tool for analyzing the structure and function of fibronectin-especially the fibronectin expressed on the surface of PMNs.
- リンク情報
- ID情報
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- ISSN : 1554-0014
- Web of Science ID : WOS:000240297400003