2021年9月
A small-molecule fluorescence probe ANP77 for sensing RNA internal loop of C, U and A/CC motifs and their binding molecules
NUCLEIC ACIDS RESEARCH
- ,
- ,
- 巻
- 49
- 号
- 15
- 開始ページ
- 8462
- 終了ページ
- 8470
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/nar/gkab650
- 出版者・発行元
- OXFORD UNIV PRESS
Small-molecules interacting with particular RNAs and modulating their functions are vital tools for RNA-targeting drug discovery. Considering the substantial distribution of the internal loops involving two contiguous cytosines opposite to a single-nucleotide base (Y/CC; Y = C, U or A) within the biologically significant functional RNAs, developing small-molecule probes targeting Y/CC sites should provide profound insight into their functions and roles in biochemical processes. Herein, we report ANP77 as the small-molecule probe for sensing RNA internal loop of Y/CC motifs and molecules binding to the motifs. The Y/CC motifs interact with ANP77 via the formation of a 1:1 complex and quench the fluorescence of ANP77. The flanking sequence-dependent binding to C/CC and U/CC sites was assessed by fluorometric screening, provided the binding heat maps. The quenching phenomena of ANP77 fluorescence was confirmed with intrinsic potential drug target pre-miR-1908. Finally, the binding-dependent fluorescence quenching of ANP77 was utilized in the fluorescence indicator displacement assay to demonstrate the potential of ANP77 as an indicator by using the RNA-binding drugs, risdiplam and branaplam.
- リンク情報
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- DOI
- https://doi.org/10.1093/nar/gkab650
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/34358308
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421207
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000697383500011&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1093/nar/gkab650
- ISSN : 0305-1048
- eISSN : 1362-4962
- PubMed ID : 34358308
- PubMed Central 記事ID : PMC8421207
- Web of Science ID : WOS:000697383500011