論文

査読有り 国際誌
2019年11月25日

Gut-derived Enterococcus faecium from ulcerative colitis patients promotes colitis in a genetically susceptible mouse host.

Genome biology
  • Jun Seishima
  • ,
  • Noriho Iida
  • ,
  • Kazuya Kitamura
  • ,
  • Masahiro Yutani
  • ,
  • Ziyu Wang
  • ,
  • Akihiro Seki
  • ,
  • Taro Yamashita
  • ,
  • Yoshio Sakai
  • ,
  • Masao Honda
  • ,
  • Tatsuya Yamashita
  • ,
  • Takashi Kagaya
  • ,
  • Yukihiro Shirota
  • ,
  • Yukako Fujinaga
  • ,
  • Eishiro Mizukoshi
  • ,
  • Shuichi Kaneko

20
1
開始ページ
252
終了ページ
252
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1186/s13059-019-1879-9

BACKGROUND: Recent metagenomic analyses have revealed dysbiosis of the gut microbiota of ulcerative colitis (UC) patients. However, the impacts of this dysbiosis are not fully understood, particularly at the strain level. RESULTS: We perform whole-genome shotgun sequencing of fecal DNA extracts from 13 healthy donors and 16 UC and 8 Crohn's disease (CD) patients. The microbiota of UC and CD patients is taxonomically and functionally divergent from that of healthy donors, with E. faecium being the most differentially abundant species between the two microbial communities. Transplantation of feces from UC or CD patients into Il10-/- mice promotes pathological inflammation and cytokine expression in the mouse colon, although distinct cytokine expression profiles are observed between UC and CD. Unlike isolates derived from healthy donors, E. faecium isolates from the feces of UC patients, along with E. faecium strain ATCC 19434, promotes colitis and colonic cytokine expression. Inflammatory E. faecium strains, including ATCC 19434 and a UC-derived strain, cluster separately from commercially available probiotic strains based on whole-genome shotgun sequencing analysis. The presence of E. faecium in fecal samples is associated with large disease extent and the need for multiple medications in UC patients. CONCLUSIONS: E. faecium strains derived from UC patients display an inflammatory genotype that causes colitis.

リンク情報
DOI
https://doi.org/10.1186/s13059-019-1879-9
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31767028
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6876129
ID情報
  • DOI : 10.1186/s13059-019-1879-9
  • PubMed ID : 31767028
  • PubMed Central 記事ID : PMC6876129

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