論文

査読有り 国際誌
2020年

Functional Analysis of Botulinum Hemagglutinin (HA).

Methods in molecular biology (Clifton, N.J.)
  • Takuhiro Matsumura
  • ,
  • Yukako Fujinaga

2132
開始ページ
191
終了ページ
200
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/978-1-0716-0430-4_20

Botulinum neurotoxin (BoNT), produced by Clostridium botulinum, is the most potent toxin and produced as a complex with non-toxic components. Food-borne botulism is caused by the ingestion of these BoNT complexes. Hemagglutinin (HA), one of the non-toxic components, is known to have lectin (carbohydrate binding) activity and E-cadherin-binding activity. These activities promote the intestinal absorption of BoNT. To elucidate the mechanism of the onset of food-borne botulism, we focused on the role of HA in the intestinal absorption of BoNT. We describe the functional analysis methods for HA, including the expression of recombinant proteins, binding to glycoproteins and epithelial cells, and localization in mouse intestinal tissue.

リンク情報
DOI
https://doi.org/10.1007/978-1-0716-0430-4_20
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32306328
ID情報
  • DOI : 10.1007/978-1-0716-0430-4_20
  • PubMed ID : 32306328

エクスポート
BibTeX RIS