2020年
Functional Analysis of Botulinum Hemagglutinin (HA).
Methods in molecular biology (Clifton, N.J.)
- ,
- 巻
- 2132
- 号
- 開始ページ
- 191
- 終了ページ
- 200
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/978-1-0716-0430-4_20
Botulinum neurotoxin (BoNT), produced by Clostridium botulinum, is the most potent toxin and produced as a complex with non-toxic components. Food-borne botulism is caused by the ingestion of these BoNT complexes. Hemagglutinin (HA), one of the non-toxic components, is known to have lectin (carbohydrate binding) activity and E-cadherin-binding activity. These activities promote the intestinal absorption of BoNT. To elucidate the mechanism of the onset of food-borne botulism, we focused on the role of HA in the intestinal absorption of BoNT. We describe the functional analysis methods for HA, including the expression of recombinant proteins, binding to glycoproteins and epithelial cells, and localization in mouse intestinal tissue.
- リンク情報
- ID情報
-
- DOI : 10.1007/978-1-0716-0430-4_20
- PubMed ID : 32306328