論文

査読有り 国際誌
2018年2月1日

A Simple and Robust Method for Culturing Human-Induced Pluripotent Stem Cells in an Undifferentiated State Using Botulinum Hemagglutinin

Biotechnology Journal
  • Mee-Hae Kim
  • ,
  • Yoshifumi Matsubara
  • ,
  • Yukako Fujinaga
  • ,
  • Masahiro Kino-oka

13
2
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/biot.201700384
出版者・発行元
Wiley-VCH Verlag

Clinical and industrial applications of human-induced pluripotent stem cells (hiPSCs) is hindered by the lack of robust culture strategies capable of sustaining a culture in an undifferentiated state. Here, a simple and robust hiPSC-culture-propagation strategy incorporating botulinum hemagglutinin (HA)-mediated selective removal of cells deviating from an undifferentiated state is developed. After HA treatment, cell–cell adhesion is disrupted, and deviated cells detached from the central region of the colony to subsequently form tight monolayer colonies following prolonged incubation. The authors find that the temporal and dose-dependent activity of HA regulated deviated-cell removal and recoverability after disruption of cell–cell adhesion in hiPSC colonies. The effects of HA are confirmed under all culture conditions examined, regardless of hiPSC line and feeder-dependent or -free culture conditions. After routine application of our HA-treatment paradigm for serial passages, hiPSCs maintains expression of pluripotent markers and readily forms embryoid bodies expressing markers for all three germ-cell layers. This method enables highly efficient culturing of hiPSCs and use of entire undifferentiated portions without having to pick deviated cells manually. This simple and readily reproducible culture strategy is a potentially useful tool for improving the robust and scalable maintenance of undifferentiated hiPSC cultures.


リンク情報
DOI
https://doi.org/10.1002/biot.201700384
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29027750
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000424099500013&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/biot.201700384
  • ISSN : 1860-7314
  • ISSN : 1860-6768
  • PubMed ID : 29027750
  • SCOPUS ID : 85033217830
  • Web of Science ID : WOS:000424099500013

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