2021年8月
PPAR gamma-Induced Global H3K27 Acetylation Maintains Osteo/Cementogenic Abilities of Periodontal Ligament Fibroblasts
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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- 巻
- 22
- 号
- 16
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.3390/ijms22168646
- 出版者・発行元
- MDPI
The periodontal ligament is a soft connective tissue embedded between the alveolar bone and cementum, the surface hard tissue of teeth. Periodontal ligament fibroblasts (PDLF) actively express osteo/cementogenic genes, which contribute to periodontal tissue homeostasis. However, the key factors maintaining the osteo/cementogenic abilities of PDLF remain unclear. We herein demonstrated that PPAR gamma was expressed by in vivo periodontal ligament tissue and its distribution pattern correlated with alkaline phosphate enzyme activity. The knockdown of PPAR gamma markedly reduced the osteo/cementogenic abilities of PDLF in vitro, whereas PPAR gamma agonists exerted the opposite effects. PPAR gamma was required to maintain the acetylation status of H3K9 and H3K27, active chromatin markers, and the supplementation of acetyl-CoA, a donor of histone acetylation, restored PPAR gamma knockdown-induced decreases in the osteo/cementogenic abilities of PDLF. An RNA-seq/ChIP-seq combined analysis identified four osteogenic transcripts, RUNX2, SULF2, RCAN2, and RGMA, in the PPAR gamma-dependent active chromatin region marked by H3K27ac. Furthermore, RUNX2-binding sites were selectively enriched in the PPAR gamma-dependent active chromatin region. Collectively, these results identified PPAR gamma as the key transcriptional factor maintaining the osteo/cementogenic abilities of PDLF and revealed that global H3K27ac modifications play a role in the comprehensive osteo/cementogenic transcriptional alterations mediated by PPAR gamma.
- リンク情報
- ID情報
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- DOI : 10.3390/ijms22168646
- PubMed ID : 34445348
- PubMed Central 記事ID : PMC8395443