2009年
Trafficking of GFP-AQP5 chimeric proteins conferred with unphosphorylated amino acids at their PKA - Target motif (152SRRTS) in MDCK-II cells
Journal of Medical Investigation
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- 巻
- 56
- 号
- 1-2
- 開始ページ
- 55
- 終了ページ
- 63
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.2152/jmi.56.55
Three constructs having mutated PKA-target motif at 152SRRTS of AQP5, an exocrine type water channel, were prepared and fused to C-terminus of green fluorescence protein cDNA to examine the effects of blocking of phosphorylation at 152SRRTS (a consensus PKA-target motif of AQP5) on translocation or trafficking of the chimeric proteins expressed in the Madin-Darby canine kidney-II (MDCK-II) cells. H-89 treatment increased translocation of wild-type GFP-AQP5 to the apical membrane. All 3 mutant molecules translocated 1.5 to 2 times more than the control wild-type GFP-AQP5. Colchicine but not cytochalasin B inhibited the translocation of wild-type GFP-AQP5. Present results suggest dephosphorylation of this consensus sequence increase GFP-AQP5 translocation, and that microtubules but not microfilaments are involved in this event.
- リンク情報
- ID情報
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- DOI : 10.2152/jmi.56.55
- ISSN : 1343-1420
- CiNii Articles ID : 80020132181
- PubMed ID : 19262015
- SCOPUS ID : 63349091431