MISC

2007年12月

Effects of prostaglandin E-1 on vascular ATP-sensitive potassium channels

JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
  • Satoru Eguchi
  • ,
  • Takashi Kawano
  • ,
  • Yinhua
  • ,
  • Katsuya Tanaka
  • ,
  • Sonoko Yasui
  • ,
  • Kazuaki Mawatari
  • ,
  • Akira Takahashi
  • ,
  • Yutaka Nakaya
  • ,
  • Shuzo Oshita
  • ,
  • Nobuyoshi Nakajo

50
6
開始ページ
686
終了ページ
691
記述言語
英語
掲載種別
DOI
10.1097/FJC.0b013e3181583d9b
出版者・発行元
LIPPINCOTT WILLIAMS & WILKINS

Background: Prostaglandin El (PGE1) has been reported to activate ATP-sensitive potassium (KATP) channels, which induces vasorelaxation. However, direct evidence of PGE1 interactions with vascular KATP channels is limited.
Methods: The present study investigated the effects and mechanisms of PGE1 on vascular KATP channels in both isometric tension and patch clamp experiments. Isometric tension experiments were performed in rat thoracic aortic rings without an endothelium. Electrophysiologic experiments were performed using patch-clamp techniques to monitor KATP channels in rat vascular smooth muscle cells.
Results: PGE1 significantly decreased the isometric tension in a concentration-dependent manner, which was partially inhibited by pretreating with a KATP channel inhibitor, glibenclamide (1 mu M), or an inhibitor of protein kinase A (PKA), Rp-cAMPS (100 mu M). Application of PGE1 to the bath solution during cell-attached recordings induced a significant increase in KATP channel activity, whereas PGE1 failed to activate KATP channels in the inside-out patches. The PGE1-induced KATP channel currents in cell-attached patches were abolished by pretreating with Rp-cAMPS (100 mu M).
Conclusions: The results indicate that the activation of vascular KATP channels played an important role in the PKA-dependent PGE1-induced vasorelaxation. Furthermore, an electrophysiological experiment demonstrated that PGE1 activated vascular KATP channels via PKA activation.

Web of Science ® 被引用回数 : 14

リンク情報
DOI
https://doi.org/10.1097/FJC.0b013e3181583d9b
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000252161300012&DestApp=WOS_CPL

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