Nov, 1992
METABOLISM OF 2,4,5,2',4',5'-HEXACHLOROBIPHENYL WITH LIVER-MICROSOMES OF PHENOBARBITAL-TREATED DOG - THE POSSIBLE FORMATION OF PCB 2,3-ARENE OXIDE INTERMEDIATE
XENOBIOTICA
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- ,
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- Volume
- 22
- Number
- 11
- First page
- 1275
- Last page
- 1290
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.3109/00498259209053156
- Publisher
- TAYLOR & FRANCIS LTD
1. Metabolism of 2,4,5,2',4',5'-hexachlorobiphenyl (HCB) was investigated in vitro using liver microsomes of one male beagle dog after phenobarbital treatment.
2. Three major metabolites were isolated and identified as 3-hydroxy-2,4,5,2',4',5'-HCB, 2-hydroxy-4,5,2',4',5'-pentachlorobiphenyl (PenCB), and 2-hydroxy-3,4,5,2',4',5'-HCB, by comparison of g.l.c.-mass spectrometry and H-1-n.m.r. data with those of authentic samples.
3. 2-Hydroxy-3,4,5,2',4',5'-HCB was found as a metabolite of 2,4,5,2',4',5'-HCB for the first time using dog liver microsomes. Present results indicate that this metabolite and the dechlorinated PenCB are derived from a metabolic intermediate, namely, 2,3-epoxy-2,4,5,2',4',5'-HCB. 2,3-Epoxide formation is a new metabolic pathway of PCB.
2. Three major metabolites were isolated and identified as 3-hydroxy-2,4,5,2',4',5'-HCB, 2-hydroxy-4,5,2',4',5'-pentachlorobiphenyl (PenCB), and 2-hydroxy-3,4,5,2',4',5'-HCB, by comparison of g.l.c.-mass spectrometry and H-1-n.m.r. data with those of authentic samples.
3. 2-Hydroxy-3,4,5,2',4',5'-HCB was found as a metabolite of 2,4,5,2',4',5'-HCB for the first time using dog liver microsomes. Present results indicate that this metabolite and the dechlorinated PenCB are derived from a metabolic intermediate, namely, 2,3-epoxy-2,4,5,2',4',5'-HCB. 2,3-Epoxide formation is a new metabolic pathway of PCB.
- Link information
- ID information
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- DOI : 10.3109/00498259209053156
- ISSN : 0049-8254
- Web of Science ID : WOS:A1992KB32400007