MISC

2009年6月

Runt-related gene 2 is involved in the inhibition of matrix metalloproteinase-13 expression by roxithromycin in human gingival epithelial cell cultures

JOURNAL OF PERIODONTAL RESEARCH
  • Seiko Tabuchi
  • Tetsuya Sakuta
  • Tohru Oyama
  • Masayuki Tokuda
  • Syouko Tatsuyama
  • Takehiro Kajihara
  • Shigetaka Nagaoka
  • Mahiro Beppu
  • Kazumasa Sugihara
  • Tetsuro Ikebe
  • Kanemitsu Shirasuna
  • Mitsuo Torii
  • 全て表示

44
3
開始ページ
283
終了ページ
288
記述言語
英語
掲載種別
DOI
10.1111/j.1600-0765.2007.01037.x
出版者・発行元
WILEY-BLACKWELL PUBLISHING, INC

Matrix metalloproteinase (MMP)-13 has wide substrate specificity compared with other MMPs and appears to be involved in periodontitis. Previously, we reported that roxithromycin (RXM) inhibits vascular endothelial growth factor expression induced by tumour necrosis factor-alpha in human periodontal ligament cells, but little is known about the effect of RXM on MMP-13 expression in human gingival epithelial cells. We therefore examined the effect of RXM on MMP-13 mRNA expression and production in cultured human gingival epithelial cells.
Human epithelial cell lines (Ca9-22, TU4, SCCTF and HSC-3) were plated in tissue culture dishes. Then, the culture supernatants and sediments were collected and the production of MMP-13 was analysed using enzyme-linked immunosorbent assay; the expression of MMP-13 mRNA and runt-related gene 2 mRNA was assessed using reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time RT-PCR. We also studied the effect of Runx2 short interfering RNA (siRNA) on the induction of MMP-13.
Roxithromycin downregulated the induction of MMP-13 in Ca9-22 cells. Roxithromycin suppressed the expression of MMP-13 mRNA not only in Ca9-22 cells, but also in other human epithelial cell lines. Roxithromycin strongly inhibited the expression of Runx2 mRNA. Furthermore, Runx2 siRNA inhibited the induction of MMP-13 in Ca9-22 cells.
These results indicate that RXM suppresses MMP-13 via the downregulation of Runx2 in human gingival epithelial cell cultures.

リンク情報
DOI
https://doi.org/10.1111/j.1600-0765.2007.01037.x
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000265556000001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1111/j.1600-0765.2007.01037.x
  • ISSN : 0022-3484
  • Web of Science ID : WOS:000265556000001

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