2001年12月1日
LC-MS-SIMの高感度の設定条件
質量分析 = Mass spectroscopy
- ,
- 巻
- 49
- 号
- 6
- 開始ページ
- 237
- 終了ページ
- 240
- 記述言語
- 日本語
- 掲載種別
- DOI
- 10.5702/massspec.49.237
- 出版者・発行元
- 一般社団法人 日本質量分析学会
For a higher sensitivity, the parameters of liquid chromatography-mass spectrometry-selected ion monitoring (LC-MS-SIM) using atmospheric pressure chemical ionization (APCI) were examined. The scan-range means the range of atomic mass unit which was scanned at the center of an established value. The sampling-point-number means the number of measurement points within the scan-range. The counting-number means the number of the data acquisition per measurement point. The area and height of a peak as sensitivity, the full width half maximum (FWHM) and the actual scan-time which was required for the scanning were measured. The plant hormone, indole-3-acetic acid (IAA) was used as a standard chemical. The suitable parameters for higher sensitivity on the measurement limit level were as follows: 1) The scan-time might correspond to the value of FWHM. 2) The counting-number was over about 7,000. 3) The scan-range might correspond to a decimal fraction of the molecular weight of the sample substance. The results showed that the value obtained from repeated sampling-point-number was a total amount, that the value obtained from repeated counting-number was a mean and that there were no loss of time on repetitions of the sampling-point-number and the counting-number.
- リンク情報
-
- DOI
- https://doi.org/10.5702/massspec.49.237
- CiNii Articles
- http://ci.nii.ac.jp/naid/10010849848
- CiNii Books
- http://ci.nii.ac.jp/ncid/AN0010555X
- ID情報
-
- DOI : 10.5702/massspec.49.237
- ISSN : 1340-8097
- CiNii Articles ID : 10010849848
- CiNii Books ID : AN0010555X
- identifiers.cinii_nr_id : 1000070271161