MISC

1998年

Characterization of the progressive sublines derived from a weakly malignant cloned cell line, ER-1, co-inoculated subcutaneously with a foreign body

CLINICAL & EXPERIMENTAL METASTASIS
  • J Hamada
  • ,
  • H Nagayasu
  • ,
  • T Kawano
  • ,
  • T Mizutani
  • ,
  • D Nakata
  • ,
  • M Hosokawa
  • ,
  • N Takeichi

16
3
開始ページ
291
終了ページ
298
記述言語
英語
掲載種別
DOI
10.1023/A:1006505211766
出版者・発行元
KLUWER ACADEMIC PUBL

We previously established an experimental model of tumor progression using a weakly malignant rat mammary carcinoma cell line, ER-1. Using this model, we demonstrated that ER-1 cells converted into highly tumorigenic and metastatic cells, ERpP, by s.c. co-inoculation with plastic plates, We here compared in vitro biological properties associated with malignancy of ER-1 cells with those of ERpP cells which were highly malignant when inoculated into syngeneic rats. In vitro growth rate of ERpP cells was higher than that of ER-1 cells under a low nutrient condition. Invasion capacity of ERpP cells to rat lung endothelial cell monolayer or reconstituted basement membrane, Matrigel, was higher than that of ER-1 cells, Migration of ERpP cells toward fibronectin or laminin was also significantly higher than that of ER-1 cells. There was no difference in gelatinolytic or plasminogen activator activity detected in conditioned media between ER-1 and ERpP cells, Furthermore, we found that ER-1 cells communicated better among themselves and with normal fibroblasts through gap junctions compared to ERpP cells. These results suggest that growth advantage in a poor nutrient condition, enhancement of cell motility, and loss or decrease of junctional communication may be associated with tumor progression of ER-1 cells. (C) 1998 Lippincott-Raven Publishers.

Web of Science ® 被引用回数 : 4

リンク情報
DOI
https://doi.org/10.1023/A:1006505211766
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000072949700010&DestApp=WOS_CPL
ID情報
  • DOI : 10.1023/A:1006505211766
  • ISSN : 0262-0898
  • Web of Science ID : WOS:000072949700010

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