MISC

1995年

ビリルビン着色歯萌出モデルLECラットの着色機序の検索-実験的黄疸との比較-

日本口腔科学会雑誌
  • 柴田敏之
  • ,
  • 渡辺一史
  • ,
  • 牧富弥代
  • ,
  • 加藤元康
  • ,
  • 永易裕樹
  • ,
  • 大森一幸
  • ,
  • 窪田正樹
  • ,
  • 小田浩範
  • ,
  • 有末真

44
3
開始ページ
392
終了ページ
398
記述言語
日本語
掲載種別
DOI
10.11277/stomatology1952.44.392
出版者・発行元
Japanese Stomatological Society

W e have previously reported that LEC (Long Evans Cinnamon) rat with spontaneous hepatitis and hepatoma is a very useful animal model for analysis of the pigmented teeth caused by bilirubin. In this study, to elucidate the mechanism of bilirubin pigmentation in LEC rat, we compared the LEC pigmented teeth with the experimental pigmented teeth caused by bile duct ligature and exogenous bilirubin administration to LEA rat, isolated from LE (Long Evance) closed colony accompanied with LEC rat, and we examined the chronological serum bilirubin and the pathological analysis of the pigmented teeth. By means of bile duct ligature of LEA rat, serum bilirubin level increased to 8.9 ± 1.1 mg/dl at the peak, but that of the pigmented teeth was not observed (0/9). As a result of this, intraperitoneal bilirubin was administered (14 mg/kg/day, for 4 days) in addition to bile duct ligature of LEA rat. Thus, serum bilirubin level increased to 13.7 ± 1.4 mg/dl at the peak and bilirubin pigmentation of the dentin was observed in 5 of 10 (5/10) rats, but there was no significant difference between the serum bilirubin level of the pigmented group and that of the non-pigmented group. Histopathological analysis of the LEA pigmented teeth using optical microscope showed that pigmentation of LEA rat was observed in incisors as a stripe in the dentin and the stripe ran parallel to the incremental line similar to that of LEC rat, and that the abnormal dentin tube was observed in the pigmentation area, but there was no correlation between abnormal dentin tube and teeth pigmentation. The microradiographic analysis disclosed enhanced permeabilities of the pigmented areas of all 5 rats, but the permeabilities of all 5 non-pigmented rats did not change. These results suggest that the hypocalcification of the matrix of the teeth is closely concerned with the bilirubin pigmentation in addition to the high level serum bilirubin.

リンク情報
DOI
https://doi.org/10.11277/stomatology1952.44.392
CiNii Articles
http://ci.nii.ac.jp/naid/10006612739
URL
https://jlc.jst.go.jp/DN/JALC/00081723140?from=CiNii
ID情報
  • DOI : 10.11277/stomatology1952.44.392
  • ISSN : 0029-0297
  • ISSN : 2185-0461
  • CiNii Articles ID : 10006612739

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