1996
Autocrine action of transforming growth factor-β1(TGF-β1) in the invasiveness of human oral squamous cell carcinoma cell lines
Journal of Oral Surgery Society of Japan
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- Volume
- 42
- Number
- 10
- First page
- 962
- Last page
- 968
- Language
- Japanese
- Publishing type
- DOI
- 10.5794/jjoms.42.962
- Publisher
- Japanese Society of Oral and Maxillofacial Surgeons
We studied the possible role of transforming growth factor-β 1 (TGF-β 1) in tumor invasiveness by analyzing the production and activation of TGF-β 1 in eight human oral squamous cell carcinoma cell lines.<BR>The rate of secretion of TGF-β 1 was measured with a [125I]-TGF-β 1 radioreceptor assay. All human oral squamous cell carcinoma cells secreted TGF-β 1 into the culture media. Three cell lines secreted very high levels of activated TGF-19.1. When the invasion of a monolayer of lung endothelial cells was assayed in vitro, highly invasive cell lines were found to secrete activated TGF-β 1 at higher rates than weakly invasive cell lines. These findings suggested that the different invasiveness of these cell lines was associated with the rate of activated TGF-β 1 production. Highly invasive potential squamous cell carcinoma cells, such as SAS, Ca 9 -22, and OSC-20, were inhibited by treatment with neutralizing anti TGF-β 1-antibody. Furthermore, the invasiveness of weakly (T. T and HSC-2) and highly (SAS) invasive cells was enhanced by SAS culture media. Affinity labeling of [125I]-TGFβ 1 to cell surface receptors revealed the two major affinity crosslinked bands (type I and II I). These experiments indicate that TGF-β may modulate the invasive potential of human oral squamous cell carcinoma cells by autocrine action.
- Link information
- ID information
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- DOI : 10.5794/jjoms.42.962
- ISSN : 0021-5163
- ISSN : 2186-1579
- CiNii Articles ID : 10011659090
- CiNii Books ID : AN00189163