2014年11月
Detection of short tandem repeat polymorphisms from human nails using direct polymerase chain reaction method
ELECTROPHORESIS
- ,
- 巻
- 35
- 号
- 21-22
- 開始ページ
- 3188
- 終了ページ
- 3192
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/elps.201400061
- 出版者・発行元
- WILEY-BLACKWELL
Human nail is an important forensic material for parental testing and individual identification in large-scale disasters. Detection of STR polymorphism from hard tissues generally requires DNA purification, which is technically complicated and time consuming. In the present study, we attempted to detect STR polymorphisms from untreated human nail samples by direct PCR amplification method using the primer mixture supplied with the GenePrint (R) SilverSTR (R) III System or the AmpFlSTR (R) Identifiler (R) PCR Amplification Kit, and Tks Gflex DNA polymerase known to be effective for amplification from crude samples. A nail fragment measuring approximately 1.5 mm in breadth and 0.5 mm in length was placed directly into a PCR tube, and various PCR conditions were tested. The PCR products were analyzed by denaturing acrylamide gel electrophoresis or CE. Multiple STR polymorphisms were detected successfully. This method that detects STR polymorphisms not only from fresh human fingernails, but also from old nail fragments stored at room temperature for up to 10 years is expected to become a novel DNA analytical method in forensic medicine and genetic studies.
- リンク情報
- ID情報
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- DOI : 10.1002/elps.201400061
- ISSN : 0173-0835
- eISSN : 1522-2683
- Web of Science ID : WOS:000345272200022