論文

査読有り
1996年7月

Structural continuity of filtration slit (slit diaphragm) to plasma membrane of podocyte

KIDNEY INTERNATIONAL
  • Y Fujigaki
  • ,
  • T Morioka
  • ,
  • K Matsui
  • ,
  • H Kawachi
  • ,
  • M Orikasa
  • ,
  • T Oite
  • ,
  • F Shimizu
  • ,
  • Batsford, SR
  • ,
  • A Vogt

50
1
開始ページ
54
終了ページ
62
記述言語
英語
掲載種別
研究論文(学術雑誌)
出版者・発行元
NATURE PUBLISHING GROUP

Murine monocional antibody 5-1-6 was reported to bind to the slit membrane and closely related structures in rat renal glomeruli; it induced heavy, reversible proteinuria and appeared to redistribute onto the plasma membrane of epithelial cells after binding at the original target sites. This phenomenon of antigenic movement has not been analyzed in detail to date. In addition to normal kidneys we also studied localization of the antigen recognized by monoclonal antibody 5-1-6 in protamine sulfate-perfused rat kidneys, in which slit diaphragms are known to be functionally modified. Isolated glomeruli as well as ultrathin kidney cryosections were labeled by the immunogold technique to clarify the relation between this antigen and the slit diaphragm. Sequential localization of injected monoclonal antibody was visualized using a post-embedding immunogold method in rats 2 hours to 12 daps after injection of antibody. Ultrastructural immunoqold labeling demonstrated that under normal conditions antigenic molecules were expressed mainly in the area beneath the slit diaphragms. Occasionally labeling was found at the base of the foot process, facing the glomerular basement membrane. After protamine sulfate treatment antigenic sites were dislocated due to the lifting and disruption of slit diaphragms, indicating that this antigen is associated with slit diaphragms. Injected antibody was localized at the filtration slits at 2 hours, and by 12 hours it had moved onto the apical plasma cell membrane of foot process. In addition, from 3 days onwards patch or cap-like formation on the plasma cell membrane of podocytes was seen. Possible shedding of antibody from podocyte cell surface membrane was occasionally encountered, but internalization of antibody was a minor event. Elution experiments in isolated glomeruli al day 3 indicated that antigen and antibody were both localized on the podocyte cell surface membrane, suggesting redistribution of immune complexes. In conclusion, filtration slits (slit diaphragms) and the apical membrane of foot process of podocytes demonstrate structural continuity as revealed by the movement of the antigen recognized by monoclonal antibody 5-1-6 as antigen-antibody complexes.

リンク情報
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1996UT64500008&DestApp=WOS_CPL
ID情報
  • ISSN : 0085-2538
  • Web of Science ID : WOS:A1996UT64500008

エクスポート
BibTeX RIS