論文

国際誌
2021年1月20日

Gan-Lu-Yin (Kanroin), Traditional Chinese Herbal Extracts, Reduces Osteoclast Differentiation In Vitro and Prevents Alveolar Bone Resorption in Rat Experimental Periodontitis

Journal of clinical medicine
  • Yuji Inagaki
  • ,
  • Jun-Ichi Kido
  • ,
  • Yasufumi Nishikawa
  • ,
  • Rie Kido
  • ,
  • Eijiro Sakamoto
  • ,
  • Mika Bando
  • ,
  • Koji Naruishi
  • ,
  • Toshihiko Nagata
  • ,
  • Hiromichi Yumoto

10
3
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/jcm10030386

Gan-Lu-Yin (GLY), a traditional Chinese herbal medicine, shows therapeutic effects on periodontitis, but that mechanism is not well known. This study aims to clarify the precise mechanism by investigating the inhibitory effects of GLY extracts on osteoclastogenesis in vitro and on bone resorption in periodontitis in vivo. RAW264.7 cells are cultured with soluble receptor activator of nuclear factor-kappa B (sRANKL) and GLY extracts (0.01-1.0 mg/mL), and stained for tartrate-resistant acid phosphatase (TRAP) to evaluate osteoclast differentiation. Experimental periodontitis is induced by placing a nylon ligature around the second maxillary molar in rats, and rats are administered GLY extracts (60 mg/kg) daily for 20 days. Their maxillae are collected on day 4 and 20, and the levels of alveolar bone resorption and osteoclast differentiation are estimated using micro-computed tomography (CT) and histological analysis, respectively. In RAW264.7 cells, GLY extracts significantly inhibit sRANKL-induced osteoclast differentiation at a concentration of more than 0.05 mg/mL. In experimental periodontitis, administering GLY extracts significantly decreases the number of TRAP-positive osteoclasts in the alveolar bone on day 4, and significantly inhibits the ligature-induced bone resorption on day 20. These results show that GLY extracts suppress bone resorption by inhibiting osteoclast differentiation in experimental periodontitis, suggesting that GLY extracts are potentially useful for oral care in periodontitis.

リンク情報
DOI
https://doi.org/10.3390/jcm10030386
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33498415
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926381
ID情報
  • DOI : 10.3390/jcm10030386
  • PubMed ID : 33498415
  • PubMed Central 記事ID : PMC7926381

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