J-GLOBAL         Last updated: Nov 29, 2018 at 19:33
Osaka University of Pharmaceutical Sciences
Faculty of Pharmaceutical Sciences
Job title
Rigaku-Hakushi (Doctor of Science)

Research Areas



Graduate School, Division of Natural Science, Kyoto University
Faculty of Science, Kyoto University

Published Papers

Cendrowski J, Lobo VJ, Sendler M, Salas A, Kühn JP, Molero X, Fukunaga R, Mayerle J, Lerch MM, Real FX
Gut   64(6) 937-947   Jun 2015   [Refereed]
Panja D, Kenney JW, D'Andrea L, Zalfa F, Vedeler A, Wibrand K, Fukunaga R, Bagni C, Proud CG, Bramham CR
Cell reports   9(4) 1430-1445   Nov 2014   [Refereed]
Eckerdt F, Beauchamp E, Bell J, Iqbal A, Su B, Fukunaga R, Lulla RR, Goldman S, Platanias LC
Oncotarget   5(18) 8442-8451   Sep 2014   [Refereed]
Chevillard-Briet M, Quaranta M, Grézy A, Mattera L, Courilleau C, Philippe M, Mercier P, Corpet D, Lough J, Ueda T, Fukunaga R, Trouche D, Escaffit F
Human molecular genetics   23(8) 2120-2131   Apr 2014   [Refereed]
Gorentla BK, Krishna S, Shin J, Inoue M, Shinohara ML, Grayson JM, Fukunaga R, Zhong XP
Journal of immunology (Baltimore, Md. : 1950)   190(3) 1026-1037   Feb 2013   [Refereed]


Y. Shi, Y. Shi, P. Frost, P. Frost, B. Hoang, B. Hoang, Y. Yang, Y. Yang, R. Fukunaga, R. Fukunaga, J. Gera, J. Gera, A. Lichtenstein, A. Lichtenstein
Oncogene   32 190-197   Jan 2013
When mTOR inhibitor rapalogs prevent cap-dependent translation of cell-cycle proteins like c-myc, continuing tumor cell growth depends on cap-independent translation, which is mediated by internal ribosome entry sites (IRESes) located in the 5′-UT...
Hironori Ogawa, Takeshi Ueda, Tomohisa Aoyama, Ami Aronheim, Shigekazu Nagata, Shigekazu Nagata, Shigekazu Nagata, Rikiro Fukunaga, Rikiro Fukunaga, Rikiro Fukunaga
Genes to Cells   8, 325-339 325-339   Apr 2003
Background: The myeloid zinc finger protein 2A (MZF-2A) is a Krüppel-type C2H2 zinc finger transcription factor expressed in myeloid cells and involved in the growth, differentiation and tumorigenesis of myeloid progenitors. Previously we identifi...
R. Fukunaga
Seikagaku. The Journal of Japanese Biochemical Society   67 265-278   Apr 1995
Hironori Ogawa, Ayako Murayama, Shigekazu Nagata, Rikiro Fukunaga
Journal of Biological Chemistry   278, 2921-2927 2921-2927   Jan 2003
The myeloid zinc finger protein (MZF)-2 is a C2H2zinc finger transcription factor that is expressed in myeloid cells and involved in the growth, differentiation, and tumorigenesis of myeloid progenitors. Here we describe a novel isoform of MZF-2, ...
Ying Hefner, Angelika G. Börsch-Haubold, Makomoto Murakami, Jonathan I. Wilde, Sophie Pasquet, David Schieltz, Farideh Ghomashchi, John R. Yates, Christopher G. Armstrong, Andrew Paterson, Philip Cohen, Rikiro Fukunaga, Tony Hunter, Ichiro Kudo, Steve P. Watson, Steve P. Watson, Michael H. Gelba, Michael H. Gelba
Journal of Biological Chemistry   275(37542) 37542-37551   Dec 2000
We have previously reported that in thrombin-stimulated human platelets, cytosolic phospholipase A2(cPLA2) is phosphorylated on Ser-505 by p38 protein kinase and on Ser-727 by an unknown kinase. Pharmacological inhibition of p38 leads to inhibitio...
Akira Kawasaki, Itaru Matsumura, Rikiro Fukunaga
Blood   96    Dec 2000
Myeloid zinc-finger protein 1 (MZF-1 ) and its splicing variant, MZF-2, are zinc-finger transcription factors that are expressed specifically in myeloid cells. In previous studies, MZF-1 has been shown to inhibit retinoic acid (RA)-induced growth ...
Yasuhiko ITO, Yoshiyuki SETO, Camilynn I. BRANNAN, Neal G. COPELAND, Nancy A. JENKINS, Rikiro FUKUNAGA, Shigekazu NAGATA
European Journal of Biochemistry   220(3) 881-891   Jan 1994
Granulocyte colony‐stimulating factor is a cytokine which specifically regulates the production of neutrophilic granulocytes. The granulocyte colony‐stimulating‐factor receptor (GCSFR) is mainly expressed in neutrophils and their precursor cells. ...
Stéphane Pyronnet, Hiroaki Imataka, Anne Claude Gingras, Rikiro Fukunaga, Rikiro Fukunaga, Tony Hunter, Nahum Sonenberg
EMBO Journal   18(270) 270-279   Jan 1999
Human eukaryotic translation initiation factor 4E (eIF4E) binds to the mRNA cap structure and interacts with eIF4G, which serves as a scaffold protein for the assembly of eIF4E and eIF4A to form the eIF4F complex. eIF4E is an important modulator o...
Wei Jiang, Gretchen Jimenez, Nicholas J. Wells, Thomas J. Hope, Geoffrey M. Wahl, Tony Hunter, Rikiro Fukunaga, Rikiro Fukunaga
Molecular Cell   2(877) 877-885   Jan 1998
We have identified a novel human protein, PRC1, that is involved in cytokinesis. PRC1 is a good substrate for several CDKs in vitro and is phosphorylated in vivo at sites that are phosphorylated by CDK in vitro, strongly suggesting that PRC1 is an...
Rikiro Fukunaga, Rikiro Fukunaga, Tony Hunter
EMBO Journal   16(1921) 1921-1933   Apr 1997
We have developed a novel expression screening method for identifying protein kinase substrates. In this method, a λ phage cDNA expression library is screened by in situ, solid-phase phosphorylation using purified protein kinase and [γ-32P]ATP. Sc...
Osamu Hiraoka, Hiroyuki Anaguchi, Kazuhiko Yamasaki, Rikiro Fukunaga, Shigekazu Nagata, Yoshimi Ota
Journal of Biological Chemistry   269 22412-22419   Sep 1994
The amino-terminal domain of the cytokine receptor homologous region (BN domain; roughly 100 amino acid residues) in the receptor for murine granulocyte colony-stimulating factor (G-CSF) was secreted as a maltose- binding protein fusion into the E...
Shigeru Saito, Rikiro Fukunaga, Motohiko Ichijo, Shigekazu Nagata
Growth Factors   10 135-143   Jan 1994
Granulocyte colony-stimulating factor (G-CSF) is a cytokine which regulates proliferation and differentiation of neutrophilic granulocytes, and its receptor (G-CSF-R) is a member of the hemopoietic growth factor receptor family. We studied the exp...
Cai Xi Pan, Rikiro Fukunaga, Shin Yonehara, Shigekazu Nagata
Journal of Biological Chemistry   268(25818) 25818-25823   Dec 1993
The mouse interleukin-3 (IL-3)-dependent hemopoietic precursor cell line 32DC13 responds to granulocyte colony-stimulating factor (G-CSF) for proliferation and differentiation. We established a subline (32D-FH) of the 32DC13 cells which has lost t...
M. Y. Lee, K. L. Fevold, K. Dorshkind, R. Fukunaga, S. Nagata, C. Rosse
Blood   82 2062-2068   Oct 1993
Transplantation of a granulocytosis-inducing murine CE mammary carcinoma into mice suppresses primary B lymphopoiesis in the marrow. The mechanisms of this tumor-induced B-cell suppression were investigated using Whitlock- Witte-type lymphoid cult...
Rikiro Fukunaga, Etsuko Ishizaka-Ikeda, Shigekazu Nagata
Cell   74(1079) 1079-1087   Sep 1993
Granulocyte colony-stimulating factor (G-CSF) is a cytokine that regulates the proliferation and differentiation of neutrophils. The G-CSF receptor (G-CSFR) is a member of the hemopoietic growth factor receptor family. A G-CSFR expression plasmid ...
Etsuko Ishizaka-Ikeda, Rikiro Fukunaga, William I. Wood, David V. Goeddel, Shigekazu Nagata
Proceedings of the National Academy of Sciences of the United States of America   90(123) 123-127   Jan 1993
The granulocyte colony-stimulating factor receptor (G-CSF-R) and growth hormone receptor (GH-R) belong to the cytokine receptor family and have some similarity in the cytokine receptor-homologous (CRH) domain of the extracellular region. Among mem...
Yoshiyuki Seto, Rikiro Fukunaga, Shigekazu Nagata, Shigekazu Nagata
Journal of Immunology   148 259-266   Jan 1992
The human chromosomal gene for the granulocyte CSF (G-CSF) receptor was molecularly cloned from a human gene library. The gene is about 16.5 kb long, and present in a single copy per haploid human genome. The human G-CSF receptor gene consists of ...
S. Nagata, R. Fukunaga
Journal of Nutritional Science and Vitaminology   38 341-344   Jan 1992
Germaine Fuh, Brian C. Cunningham, Rikiro Fukunaga, Shigekazu Nagata, David V. Goeddel, James A. Wells
Science   256 1677-1680   Jan 1992
A hybrid receptor was constructed that contained the extracellular binding domain of the human growth hormone (hGH) receptor linked to the transmembrane and intracellular domains of the murine granulocyte colony-stimulating factor receptor. Additi...
Rikiro Fukunaga, Etsuko Ishizaka-Ikeda, Cai Xi Pan, Yoshiyuki Seto, Shigekazu Nagata
EMBO Journal   10(2855) 2855-2865   Dec 1991
The granulocyte colony-stimulating factor (G-CSF) receptor has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular r...
Minako Y. Lee, Rikiro Fukunaga, Theodore J. Lee, Joan L. Lottsfeldt, Shigekazu Nagata
Blood   77 2135-2141   May 1991
To understand the etiology of bone modulation and hypercalcemia observed in granulocytosis of a tumor-bearing animal model and to gain insight into the implication of sustained hematopoietic stimulation on the bone tissue, in vivo responses of nor...
Tatsuo Kinashi, Kwang Ho Lee, Masako Ogawa, Kaoru Tohyama, Kei Tashiro, Rikiro Fukunaga, Shigekazu Nagata, Tasuku Honjo, Tasuku Honjo
Journal of Experimental Medicine   173 1267-1279   May 1991
We are interested to know whether expression of a lineage-specific growth factor receptor is deterministic to lineage commitment during hematopoiesis. For this purpose, we introduced the human c-fms gene into the multipotential stem cell clone LyD...
Shigekazu Nagata, Rikiro Fukunaga
Progress in Growth Factor Research   3 131-141   Jan 1991
Granulocyte colony-stimulating factor (G-CSF) is a glycoprotein of Mrof about 20,000, which stimulates proliferation and differentiation of progenitor cells of neutrophils. Recent clinical application of G-CSF has proven that this hormone is effec...
Johji Inazawa, Rikiro Fukunaga, Yoshiyuki Seto, Hitoshi Nakagawa, Shinichi Misawa, Tatsuo Abe, Shigekazu Nagata
Genomics   10 1075-1078   Jan 1991
The gene for the granulocyte colony-stimulating factor (G-CSF) receptor (CSF3R) was localized on the p35-p34.3 region of human chromosome 1 by in situ hybridization using human G-CSF receptor cDNA as the probe. Polymerase chain reaction using olig...
Yuji Isegawa, Yoshiaki Fujiwara, Yoshiaki Fujiwara, Yoshiaki Fujiwara, Atsushi Ohshima, Rikiro Fukunaga, Hiroshi Murakami, Koichi Yamanishi, Yoshihiro Sokawa
Nucleic Acids Research   18 4936   Aug 1990
Rikiro Fukunaga, Etsuko Ishizaka-Ikeda, Shigekazu Nagata
Journal of Biological Chemistry   265(14008) 14008-14015   Aug 1990
A receptor for mouse granulocyte colony-stimulating factor (G-CSF) has been found on the cell surface of mouse myeloid leukemia cell line NFS-60. Chemical cross-linking of the receptor with radioiodinated G-CSF, followed by gel electrophoresis in ...
Rikiro Fukunaga, Etsuko Ishizaka-Ikeda, Yoshiyuki Seto, Shigekazu Nagata
Cell   61(341) 341-350   Apr 1990
Two cDNAs encoding the receptor for murine granulocyte colony-stimulating factor (G-CSF) were isolated from a CDM8 expression library of mouse myeloid leukemia NFS-60 cells, and their nucleotide sequences were determined. Murine G-CSF receptor exp...
Granulocyte colony-stimulating factor receptor and its related receptors.
Growth Factors   8 99   1993
Rikiro Fukunaga, Yoshiyuki Seto, Seiichi Mizushima, Shigekazu Nagata
Proceedings of the National Academy of Sciences of the United States of America   87(8702) 8702-8706   Jan 1990
Three cDNAs for the human granulocyte colony-stimulating factor (G-CSF) receptor were isolated from the cDNA libraries of human U937 leukemia cells and placenta by using a murine G-CSF receptor cDNA as the probe. The human G-CSF receptor containin...
Identifying MAPK substrates by expression screening with solid-phase phosphorylation
Methods Mol. Biol.   250, 211-236    2004
Yoshitake Ichii, Rikiro Fukunaga, Satoshi Shiojiri, Yoshihiro Sokawa
Nucleic Acids Research   14 10117   Dec 1986
Rikiro Fukunaga, Masako Matsuyama, Masako Matsuyama, Hamki Okamura, Hamki Okamura, Kumiko Nagata, Kumiko Nagata, Shigekazu Nagata, Shigekazu Nagata, Yoshihiro Sokawa
Nucleic Acids Research   14 4421-4436   Jun 1986
The relative levels of DNA methylation at CCGG sequences within and around the interferon-γ (IFN-γ) gene in normal human tissues and cell lines were examined by Southern blot analysis using isoschizomeric restriction enzymes, HpaII and MspI. On th...
Kazuo Tsukui, Rikiro Fukunaga, Keizo Ito
MICROBIOLOGY and IMMUNOLOGY   30 1271-1279   Jan 1986
Two monoclonal antibodies against human IFN-α—one against natural leukocyte IFN-α and the other against recombinant human IFN-a 2 produced in E. coli—were prepared, and designated as HT-1, and 104-5-f, respectively. These monoclonal antibodies wer...
Satoshi Shiojiri, Rikiro Fukunaga, Yoshitake Ichii, Yoshihiro Sokawa
Journal of Biochemistry   99 1455-1464   Jan 1986
17S po1y(A)+which hybridized to an oligonucleotide complementary to a part of the partial cDNA (ElcDNA) (Merlin et al. (1983) Proc. Natl Acad. Sci. U.S. 80, 4904) for 2-5A synthetase, was isolated from interferon-treated human KB cells and used fo...
Hajime Nagashima, Rikiro Fukunaga, Masako Matsuyama, Yoshihiro Sokawa
MICROBIOLOGY and IMMUNOLOGY   30 1003-1010   Jan 1986
The cytoplasmic dot hybridization method was improved by eliminating non-specific false signals. The non-specific binding to a probe disappeared almost completely when the hybridized nitrocellulose sheet was treated with pronase. Using this improv...
R. Fukunaga, Y. Sokawa, S. Nagata
Proceedings of the National Academy of Sciences of the United States of America   81 5086-5090   Jan 1984
Mouse C127I cells were transformed with a chimeric plasmid consisting of bovine papillomavirus and human interferon (HuIFN) gene (either IFN-γ or IFN-α5) placed under the control of simian virus 40 early promoter. The transformed cells retained 30...

Research Grants & Projects

Mechanism of Intracellular Signal Transduction Mediated by Protein Kinases
Molecular Mechanism for Hematopoiesis