MISC

2013年6月

Neutralizing Antibody Against Granulocyte/Macrophage Colony-Stimulating Factor Inhibits Inflammatory Response in Experimental Otitis Media

LARYNGOSCOPE
  • Shin Kariya
  • ,
  • Mitsuhiro Okano
  • ,
  • Takaya Higaki
  • ,
  • Seiichiro Makihara
  • ,
  • Takenori Haruna
  • ,
  • Motoharu Eguchi
  • ,
  • Kazunori Nishizaki

123
6
開始ページ
1514
終了ページ
1518
記述言語
英語
掲載種別
DOI
10.1002/lary.23795
出版者・発行元
WILEY

Objectives/Hypothesis: Granulocyte/macrophage colony-stimulating factor is important in the pathogenesis of acute and chronic inflammatory disease. We hypothesized that granulocyte/macrophage colony-stimulating factor plays a pivotal role in middle ear inflammation and that neutralization of granulocyte/macrophage colony-stimulating factor would inhibit neutrophil migration into the middle ear and production of inflammatory mediators.
Study Design: Animal experiment.
Methods: We used transtympanic administration of lipopolysaccharide, a major component of gram-negative bacteria, into mice to induce an experimental otitis media. Control mice received injection of phosphate-buffered saline into the middle ear cavity. Mice were systemically treated with granulocyte/macrophage colony-stimulating factor neutralizing antibody or control immunoglobulin G via intraperitoneal injection 2 hours before transtympanic injection of lipopolysaccharide or phosphate-buffered saline. Middle ear effusions were collected. Concentrations of interleukin (IL)-1 beta, tumor necrosis factor (TNF)-alpha, keratinocyte chemoattractant, and macrophage inflammatory protein-2 in middle ear effusions were measured by enzyme-linked immunosorbent assay. Histologic examination of the middle ear was also performed.
Results: Transtympanic injection of lipopolysaccharide upregulated levels of granulocyte/macrophage colony-stimulating factor, IL-1 beta, TNF-alpha, keratinocyte chemoattractant, and macrophage inflammatory protein-2 in the middle ear. Concentrations of cytokines and chemokines were significantly decreased in mice injected with granulocyte/macrophage colony-stimulating factor neutralizing antibody. Infiltration of inflammatory cells into the middle ear cavity induced by lipopolysaccharide was also significantly reduced by neutralization of granulocyte/macrophage colony-stimulating factor.
Conclusions: Systemic injection of granulocyte/macrophage colony-stimulating factor neutralizing antibody inhibits the middle ear inflammation induced by lipopolysaccharide in mice. Our findings suggest that granulocyte/macrophage colony-stimulating factor may offer a novel therapeutic target for the management of intractable otitis media.

Web of Science ® 被引用回数 : 8

リンク情報
DOI
https://doi.org/10.1002/lary.23795
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000320784000036&DestApp=WOS_CPL

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