MISC

2013年9月

The engraftment and differentiation of transplanted bone marrow-derived cells in the olfactory bulb after methimazole administration

Acta Oto-Laryngologica
  • Yohei Noda
  • ,
  • Kazunori Nishizaki
  • ,
  • Junko Yoshinobu
  • ,
  • Yorihisa Orita
  • ,
  • Hidetsugu Tsujigiwa
  • ,
  • Masao Yamada

133
9
開始ページ
951
終了ページ
956
記述言語
英語
掲載種別
DOI
10.3109/00016489.2013.803153

Conclusion: Bone marrow-derived cells can be engrafted in the olfactory bulb and a few cells can differentiate into mitral/tufted cells in the olfactory bulb. Objectives: To investigate whether bone marrow-derived cells can be engrafted into the olfactory bulb and differentiate into neurons and glial cells after methimazole administration. Methods: Bone marrow of GFP (green fluorescence protein) mice was transplanted into lethally irradiated recipient mice. Immunostaining was performed to confirm the cell types of bone marrow-derived cells expressing GFP. Results: GFP-positive cells were observed in the olfactory bulb at 2 days after methimazole administration. The number of dendritic GFP-positive cells increased up to 30 days after methimazole administration and then decreased. Double immunostaining for GFP and Iba1 or TBX21 showed that a large population of the GFP-positive cells had characteristics of microglia/macrophages and a few cells had characteristics of mitral/tufted cells. © 2013 Informa Healthcare.

リンク情報
DOI
https://doi.org/10.3109/00016489.2013.803153
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23822107

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