MISC

2014年12月

Cellular Responses to Staphylococcus aureus Alpha-Toxin in Chronic Rhinosinusitis with Nasal Polyps

ALLERGOLOGY INTERNATIONAL
  • Mitsuhiro Okano
  • ,
  • Tazuko Fujiwara
  • ,
  • Shin Kariya
  • ,
  • Takaya Higaki
  • ,
  • Takenori Haruna
  • ,
  • Osamu Matsushita
  • ,
  • Yohei Noda
  • ,
  • Seiichiro Makihara
  • ,
  • Kengo Kanai
  • ,
  • Yasuyuki Noyama
  • ,
  • Masami Taniguchi
  • ,
  • Kazunori Nishizaki

63
4
開始ページ
563
終了ページ
573
記述言語
英語
掲載種別
DOI
10.2332/allergolint.14-OA-0703
出版者・発行元
JAPANESE SOCIETY ALLERGOLOGY

Background: In contrast to Staphylococcus aureus-derived superantigenic exotoxins, the role of non-superantigenic exotoxins in the pathogenesis of eosinophilic airway diseases remains obscure. We sought to characterize S. aureus alpha-toxin-induced cellular responses in chronic rhinosinusitis with nasal polyps (CRSwNP).
Methods: Dispersed nasal polyp cells and uncinate tissue cells were prepared from patients with CRS with and without nasal polyps, respectively. Cells were incubated with various concentrations of alpha-toxin or staphylococcal enterotoxin B and then the levels of IL-5, IL-13, IFN-gamma, IL-17A, and IL-10 in the cell supernatants were determined. The pathophysiological significance of alpha-toxin-induced cytokine production was also determined including radiological severity of rhinosinusitis, tissue and blood eosinophilia, serum total IgE level, and 1-s forced expiratory volume/forced vital capacity ratio (FEV1/FVC).
Results: Nasal polyp cells produced substantial amounts of IL-5, IL-13, IFN-gamma, IL-17A, and IL-10 in response to alpha-toxin. Cytokine production was higher in nasal polyp cells than in uncinate tissue cells. The potency of alpha-toxin in stimulating IL-5, IL-13, and IL-10 production was comparable to that of enterotoxin. Alpha-toxin-induced IFN-gamma, IL-17A, and IL-10 production significantly and negatively correlated with the degree of eosinophil infiltration into nasal polyps. Conversely, alpha-toxin-induced IFN-gamma and IL-10 production significantly and positively correlated with FEV1/FVC. IL-10 production was significantly lower in asthmatic patients compared to non-asthmatics
Conclusions: S. aureus-derived alpha-toxin can provoke cellular responses in nasal polyps. These responses, especially failure to synthesize IL-10, may play a role in the pathophysiology of CRSwNP.

Web of Science ® 被引用回数 : 11

リンク情報
DOI
https://doi.org/10.2332/allergolint.14-OA-0703
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000347359700008&DestApp=WOS_CPL

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