MISC

2015年8月

Staphylococcal protein A-formulated immune complexes suppress enterotoxin-induced cellular responses in nasal polyps

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
  • Mitsuhiro Okano
  • ,
  • Tazuko Fujiwara
  • ,
  • Shin Kariya
  • ,
  • Takenori Haruna
  • ,
  • Takaya Higaki
  • ,
  • Yasuyuki Noyama
  • ,
  • Sei-ichiro Makihara
  • ,
  • Kengo Kanai
  • ,
  • Kazunori Nishizaki

136
2
開始ページ
343
終了ページ
+
記述言語
英語
掲載種別
DOI
10.1016/j.jaci.2014.10.058
出版者・発行元
MOSBY-ELSEVIER

Background: Recent studies have revealed that Staphylococcus aureus and its components participate in the pathogenesis of eosinophilic airway diseases, such as chronic rhinosinusitis with nasal polyps.
Objective: We sought to determine whether staphylococcal protein A (SpA) from S aureus regulated cellular responses in nasal polyps, especially when coupled to immunoglobulins in immune complexes (ICs).
Methods: Dispersed nasal polyp cells (DNPCs) or peripheral blood monocytes were cultured in vitro with SpA in the presence or absence of IgG, and IL-5, IL-13, IFN-gamma, IL-17A, and IL-10 levels were measured in the supernatants. The effect of SpA exposure on staphylococcal enterotoxin B-induced cytokine production by DNPCs in the presence and absence of IgG, IgA, and autologous serum was also examined.
Results: Exposure to SpA induced DNPCs to produce significantly higher IL-10, IL-13, and IL-17A levels than DNPCs without SpA, although the magnitude of the IL-17A increase was less than that of IL-10 and IL-13. SpA induced IL-10 production mainly from adherent DNPCs, and this was significantly enhanced in the presence of IgG; similar results were observed in peripheral blood monocytes. IC formation between SpA and IgG (SpA-IgG ICs) was confirmed by using native polyacrylamide gel electrophoresis. SpA-IgG ICs, but not SpA alone, almost completely suppressed staphylococcal enterotoxin B-induced IL-5, IL-13, IFN-gamma, and IL-17A production by DNPCs; similar inhibition was observed in DNPCs treated with SpA in the presence of either IgA or autologous serum.
Conclusions: Our results suggest that SpA can regulate the pathogenesis of enterotoxin-induced inflammation in patients with chronic rhinosinusitis with nasal polyps through coupling to immunoglobulins.

Web of Science ® 被引用回数 : 8

リンク情報
DOI
https://doi.org/10.1016/j.jaci.2014.10.058
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000359004900015&DestApp=WOS_CPL

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