MISC

2016年10月

Expression of macrophage migration inhibitory factor and CD74 in the inner ear and middle ear in lipopolysaccharide-induced otitis media

ACTA OTO-LARYNGOLOGICA
  • Hisashi Ishihara
  • ,
  • Shin Kariya
  • ,
  • Mitsuhiro Okano
  • ,
  • Pengfei Zhao
  • ,
  • Yukihide Maeda
  • ,
  • Kazunori Nishizaki

136
10
開始ページ
1011
終了ページ
1016
記述言語
英語
掲載種別
DOI
10.1080/00016489.2016.1179786
出版者・発行元
TAYLOR & FRANCIS LTD

Conclusion: Significant expression of macrophage migration inhibitory factor and its receptor (CD74) was observed in both the middle ear and inner ear in experimental otitis media in mice. Modulation of macrophage migration inhibitory factor and its signaling pathway might be useful in the management of inner ear inflammation due to otitis media.Objectives: Inner ear dysfunction secondary to otitis media has been reported. However, the specific mechanisms involved are not clearly understood. The aim of this study is to investigate the expression of macrophage migration inhibitory factor and CD74 in the middle ear and inner ear in lipopolysaccharide-induced otitis media.Method: BALB/c mice received a transtympanic injection of either lipopolysaccharide or phosphate-buffered saline (PBS). The mice were sacrificed 24h after injection, and temporal bones were processed for polymerase chain reaction (PCR) analysis, histologic examination, and immunohistochemistry.Results: PCR examination revealed that the lipopolysaccharide-injected mice showed a significant up-regulation of macrophage migration inhibitory factor in both the middle ear and inner ear as compared with the PBS-injected control mice. The immunohistochemical study showed positive reactions for macrophage migration inhibitory factor and CD74 in infiltrating inflammatory cells, middle ear mucosa, and inner ear in the lipopolysaccharide-injected mice.


リンク情報
DOI
https://doi.org/10.1080/00016489.2016.1179786
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000384212000005&DestApp=WOS_CPL
ID情報
  • DOI : 10.1080/00016489.2016.1179786
  • ISSN : 0001-6489
  • eISSN : 1651-2251
  • Web of Science ID : WOS:000384212000005

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