MISC

2016年10月

Effect of prostaglandin D2 on VEGF release by nasal polyp fibroblasts

ALLERGOLOGY INTERNATIONAL
  • Kengo Kanai
  • ,
  • Mitsuhiro Okano
  • ,
  • Tazuko Fujiwara
  • ,
  • Shin Kariya
  • ,
  • Takenori Haruna
  • ,
  • Ryotaro Omichi
  • ,
  • Sei-ichiro Makihara
  • ,
  • Yuji Hirata
  • ,
  • Kazunori Nishizaki

65
4
開始ページ
414
終了ページ
419
記述言語
英語
掲載種別
DOI
10.1016/j.alit.2016.03.003
出版者・発行元
JAPANESE SOCIETY ALLERGOLOGY

Background: Vascular endothelial growth factor (VEGF) is known to be associated with the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). VEGF is produced by a variety of cells including fibroblasts. It was recently reported that prostaglandin (PG) E-2 induces VEGF release by nasal polyp fibroblasts. However, little is known regarding possible regulation of VEGF by other PGs. We have reported that molecules that regulate PGD(2) metabolism play roles in the pathogenesis of CRS including in local eosinophilia and type 2 cytokine production. In the present study, we sought to determine whether PGD(2) regulates VEGF release by nasal polyp fibroblasts.
Methods: Nasal polyp fibroblasts were established from nasal polyps. These fibroblasts were stimulated with serial dilutions of PGD(2) or PGD(2) receptor (DP/CRTH2)-selective agonists in the presence or absence of receptor-selective antagonists. The concentration of VEGF in the culture supernatants was determined using ELISA.
Results: 5 mM of PGD(2) significantly induced VEGF release by nasal polyp fibroblasts. VEGF release was also obtained by stimulation with a DP receptor-selective, but not with a CRTH2 receptor-selective agonist. In addition, PGD(2)-induced VEGF release was significantly inhibited by pre-treatment with DP receptor-selective antagonists. In contrast, pre-treatment with a CRTH2 receptor-selective antagonist significantly enhanced PGD(2)-induced VEGF release.
Conclusions: PGD(2) stimulates VEGF production via DP but not CRTH2 receptors in nasal polyp fibroblasts. Copyright (C) 2016, Japanese Society of Allergology. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license.


リンク情報
DOI
https://doi.org/10.1016/j.alit.2016.03.003
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000385500400010&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.alit.2016.03.003
  • ISSN : 1323-8930
  • eISSN : 1440-1592
  • Web of Science ID : WOS:000385500400010

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