MISC

2009年4月

Mobiloization of intracellular calcium ions in chicken and rat lymphocytes induced by T cell mitogens

ANIMAL SCIENCE JOURNAL
  • Gerilechaogetu
  • ,
  • Kiyoaki Narahara
  • ,
  • Asaki Abe
  • ,
  • Yasuhiro Kondo

80
2
開始ページ
163
終了ページ
170
記述言語
英語
掲載種別
DOI
10.1111/j.1740-0929.2008.00608.x
出版者・発行元
WILEY-BLACKWELL PUBLISHING, INC

Cytosolic Ca(2+) is known to be an important factor in intracellular signaling pathways that regulate several cellular functions. The present study was designed to measure the intracellular concentrations of Ca(2+) ([Ca(2+)](i)) in T cell mitogen-stimulated chicken lymphocytes, and to compare the results with those in rat lymphocytes. [Ca(2+)](i) was increased in the thymocytes, splenocytes and bursacytes of chickens, and in the thymocytes and splenocytes of rats following exposure to the mitogens phytohaemagglutinin (PHA) and concanavalin A (ConA). Increases were greatest in the thymocytes followed by the splenocytes and bursacytes. The PHA-induced changes in the thymocytes and splenocytes were similar in chickens and rats, but the ConA-induced increases were significantly lower in the chickens than rats. Pretreatment with EGTA before the application of PHA and ConA completely suppressed the rise in [Ca(2+)](i) in all the chicken lymphocytes, indicating that the increases that occurred in PHA- and ConA-treated chicken lymphocytes could be entirely attributed to the influx of extracellular Ca(2+). On the other hand, the PHA- and ConA-induced increase in [Ca(2+)](i) in rat lymphocytes was not completely suppressed by EGTA, indicating the recruitment of Ca(2+) from the intracellular Ca(2+) pool. The results suggest species differences in the Ca(2+)-based responses to T cell mitogens between chicken lymphocytes and rat lymphocytes.

リンク情報
DOI
https://doi.org/10.1111/j.1740-0929.2008.00608.x
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000264344400007&DestApp=WOS_CPL
ID情報
  • DOI : 10.1111/j.1740-0929.2008.00608.x
  • ISSN : 1344-3941
  • Web of Science ID : WOS:000264344400007

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