2009年4月
Mobiloization of intracellular calcium ions in chicken and rat lymphocytes induced by T cell mitogens
ANIMAL SCIENCE JOURNAL
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- 巻
- 80
- 号
- 2
- 開始ページ
- 163
- 終了ページ
- 170
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1111/j.1740-0929.2008.00608.x
- 出版者・発行元
- WILEY-BLACKWELL PUBLISHING, INC
Cytosolic Ca(2+) is known to be an important factor in intracellular signaling pathways that regulate several cellular functions. The present study was designed to measure the intracellular concentrations of Ca(2+) ([Ca(2+)](i)) in T cell mitogen-stimulated chicken lymphocytes, and to compare the results with those in rat lymphocytes. [Ca(2+)](i) was increased in the thymocytes, splenocytes and bursacytes of chickens, and in the thymocytes and splenocytes of rats following exposure to the mitogens phytohaemagglutinin (PHA) and concanavalin A (ConA). Increases were greatest in the thymocytes followed by the splenocytes and bursacytes. The PHA-induced changes in the thymocytes and splenocytes were similar in chickens and rats, but the ConA-induced increases were significantly lower in the chickens than rats. Pretreatment with EGTA before the application of PHA and ConA completely suppressed the rise in [Ca(2+)](i) in all the chicken lymphocytes, indicating that the increases that occurred in PHA- and ConA-treated chicken lymphocytes could be entirely attributed to the influx of extracellular Ca(2+). On the other hand, the PHA- and ConA-induced increase in [Ca(2+)](i) in rat lymphocytes was not completely suppressed by EGTA, indicating the recruitment of Ca(2+) from the intracellular Ca(2+) pool. The results suggest species differences in the Ca(2+)-based responses to T cell mitogens between chicken lymphocytes and rat lymphocytes.
- リンク情報
- ID情報
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- DOI : 10.1111/j.1740-0929.2008.00608.x
- ISSN : 1344-3941
- Web of Science ID : WOS:000264344400007