2018年2月21日
Affinity resins as new tools for identifying target proteins of ascorbic acid
Analyst
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- 巻
- 143
- 号
- 4
- 開始ページ
- 874
- 終了ページ
- 882
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1039/c7an01592e
- 出版者・発行元
- Royal Society of Chemistry
l-Ascorbic acid (AA) has diverse physiological functions, but little is known about the functional mechanisms of AA. In this study, we synthesized two types of affinity resin on which AA is immobilized in a stable form to identify new AA-targeted proteins, which can provide important clues for elucidating unknown functional mechanisms of AA. To our knowledge, an affinity resin on which AA as a ligand is immobilized has not been prepared, because AA is very unstable and rapidly degraded in an aqueous solution. By using the affinity resins, cytochrome c (cyt c) was identified as an AA-targeted protein, and we showed that oxidized cyt c exhibits specific affinity for AA. These results suggest that two kinds of AA-affinity resin can be powerful tools to identify new target proteins of AA.
- リンク情報
- ID情報
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- DOI : 10.1039/c7an01592e
- ISSN : 1364-5528
- ISSN : 0003-2654
- eISSN : 1364-5528
- PubMed ID : 29327754
- SCOPUS ID : 85041956437