論文

査読有り
2013年4月

Tissue redox activity as a sensing platform for imaging of cancer based on nitroxide redox cycle

EUROPEAN JOURNAL OF CANCER
  • Zhivko Zhelev
  • ,
  • Ichio Aoki
  • ,
  • Veselina Gadjeva
  • ,
  • Biliana Nikolova
  • ,
  • Rumiana Bakalova
  • ,
  • Tsuneo Saga

49
6
開始ページ
1467
終了ページ
1478
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.ejca.2012.10.026
出版者・発行元
ELSEVIER SCI LTD

The experience in free radical biology and medicine shows the crucial role of redox signalling in carcinogenesis. The cells and tissues of healthy mammals are characterised by a low level of reactive oxygen species (ROS) and some constant (reference) level of reducing equivalents. Increasing of ROS above the critical level provokes genomic instability. The present study describes universal methodology for direct imaging of tissue redox activity in carcinogenesis, which allows a differentiation of cancer development from normal condition. The experiments were conducted on: neuroblastoma-bearing mice; colon cancer-bearing mice; and healthy mice. The tissue redox activity was visualised in vivo by nitroxide-enhanced magnetic resonance imaging (MRI) on anesthetised animals. The method is based on nitroxide redox cycle, coupled with appearance/disappearance of MRI signal. The half-life (tau(1/2)) of nitroxide-enhanced MRI signal in the respective tissue was used as a diagnostic marker. The study provides direct evidence that healthy and cancer-bearing mammalian tissues are characterised by different redox activities - a basis for cancer diagnosis. The tissues (cancer and 'normal') of cancer-bearing mammals were characterised by a long-lived MRI signal (tau(1/2) > 14 min), indicating a high oxidative activity. The tissues of healthy organism were characterised by a short-lived MRI signal (tau(1/2) = 1-3 min), indicating a high reducing activity. The study shows that tissue redox activity is a sensing platform for imaging of cancer using nitroxide-enhanced MRI. It also suggests that 'normal' tissues of cancer-bearing organism are susceptible to oxidative damage. (C) 2012 Elsevier Ltd. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.ejca.2012.10.026
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000317188600031&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.ejca.2012.10.026
  • ISSN : 0959-8049
  • Web of Science ID : WOS:000317188600031

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