論文

査読有り
2012年7月

Novel human monoclonal antibody against epidermal growth factor receptor as an imaging probe for hepatocellular carcinoma

NUCLEAR MEDICINE COMMUNICATIONS
  • Chizuru Sogawa
  • Atsushi B. Tsuji
  • Chisato Yoshida
  • Masayuki Inubushi
  • Takako Furukawa
  • Mitsuru Koizumi
  • Yasushi Akahori
  • Yoshinori Ukai
  • Gene Kurosawa
  • Yoshikazu Kurosawa
  • Tsuneo Saga
  • 全て表示

33
7
開始ページ
719
終了ページ
725
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1097/MNM.0b013e3283531d68
出版者・発行元
LIPPINCOTT WILLIAMS & WILKINS

Objective The epidermal growth factor receptor (EGFR) is overexpressed in many epithelial cancers, including hepatocellular carcinoma (HCC), and is an attractive target for cancer imaging and therapy. We attempted a novel noninvasive imaging method to evaluate anti-EGFR human monoclonal antibody clones for determining the uptake of therapeutic anti-EGFR antibody in HCC.
Methods In-vitro cell binding of nine I-125-labeled antibody clones was compared in the human epidermoid cancer cell line A431, in three HCC cell lines Hep-G2, SK-Hep1, and HuH-7, and in the EGFR-negative control cell line A4. In-111-labeled or I-125-labeled 048-006 was subjected to cell binding, competitive inhibition, and internalization assays using A431, SK-Hep1, and HuH-7. Further, In-111-labeled 048-006 was evaluated in in-vivo biodistribution analysis and single-photon imaging in nude tumor-bearing mice.
Results The 048-006 clone showed the highest binding to EGFR-expressing cells among the nine antibodies. In-111-labeled or I-125-labeled 048-006 specifically bound to EGFR-expressing cells with high affinity and was internalized after binding to EGFR. A431 and HuH-7 tumors showed high In-111-labeled 048-006 uptake, which was visualized by single-photon imaging.
Conclusion Radiolabeled human anti-EGFR monoclonal antibody 048-006 has the potential to be a safer imaging probe for predicting tumor uptake of anti-EGFR antibody therapeutic agents in HCC. Nucl Med Commun 33:719-725 (C) 2012 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.

リンク情報
DOI
https://doi.org/10.1097/MNM.0b013e3283531d68
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000305500600006&DestApp=WOS_CPL
ID情報
  • DOI : 10.1097/MNM.0b013e3283531d68
  • ISSN : 0143-3636
  • Web of Science ID : WOS:000305500600006

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