論文

査読有り
1998年1月7日

Avidin targeting of intraperitoneal tumor xenografts

Journal of the National Cancer Institute
  • Zhengsheng Yao
  • ,
  • Meili Zhang
  • ,
  • Harumi Sakahara
  • ,
  • Tsuneo Saga
  • ,
  • Yasushi Arano
  • ,
  • Junji Konishi

90
1
開始ページ
25
終了ページ
29
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/jnci/90.1.25
出版者・発行元
Oxford University Press

Background: Lectins (proteins that bind specific sugar molecules on glycoproteins and glycolipids) are expressed at various levels on the surface of tumor cells. Conjugation of cytotoxic agents to glycoproteins recognized by lectins could be useful in the treatment of tumors. Avidin (a highly glycosylated, positively charged protein found in egg white) contains terminal N-acetylglucosamine and mannose residues that bind to some lectins. In this study, we tested the ability of avidin, labeled through conjugation to radioactive biotin (a B vitamin), to target intraperitoneal tumors. Methods: Biotin was radioactively labeled with 111In. Four tumor models (one ovarian, one lung, and two colon) were established in nude mice by intraperitoneal injection of cultured cancer cells. The following two approaches were used in the intraperitoneal administration of avidin: 1) radioactive biotin-avidin conjugates were injected and 2) avidin was injected 1-24 hours before the injection of radioactive biotin (avidin pretargeting
avidin-biotin conjugates formed in vivo). The distribution of injected radioactivity in the tissues of treated animals was assessed. Results: Radiolabeled avidin localized highly and rapidly in the tumors. More than 50% of the administered dose of avidin-biotin conjugate accumulated per gram of tumor tissue 2 hours after injection
high tumor uptake of radioactivity was observed up to 24 hours after conjugate injection. In contrast, accumulation of radioactivity in normal tissues was low, yielding high tumor to nontumor ratios. With avidin pretargeting, accumulation of radioactivity in the liver, kidney, and spleen was reduced to a greater extent than that in the tumor, and tumor to nontumor ratios were increased. Conclusions: Avidin may be a promising vehicle for the delivery of radioisotopes, drugs, toxins, or therapeutic genes to intraperitoneal tumors.

リンク情報
DOI
https://doi.org/10.1093/jnci/90.1.25
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/9428779
ID情報
  • DOI : 10.1093/jnci/90.1.25
  • ISSN : 0027-8874
  • PubMed ID : 9428779
  • SCOPUS ID : 0032491807

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