Mar, 2012
Successive analysis of antigen trapping and enzymatic digestion on membrane-immobilized avidin
ANALYTICAL BIOCHEMISTRY
- ,
- Volume
- 422
- Number
- 1
- First page
- 55
- Last page
- 57
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1016/j.ab.2011.12.023
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
Avidin from egg white was migrated toward a cathode of nondenaturing electrophoresis and then immobilized on a polyvinylidene difluoride membrane. Adrenocorticotropic hormone (ACTH) was specifically captured after the biotinylated anti-ACTH antibody was bound to the membrane-immobilized avidin, and the captured ACTH was digested by the biotinylated trypsin on the membrane after extraction. The digested polypeptides from the ACTH were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). These results indicate that target substances can be specifically trapped and digested on membrane-immobilized avidin. (C) 2011 Elsevier Inc. All rights reserved.
- Link information
- ID information
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- DOI : 10.1016/j.ab.2011.12.023
- ISSN : 0003-2697
- Web of Science ID : WOS:000300759500010