論文

査読有り
2017年9月

Characterization of a novel allergenic protein from the octocoral Scleronephthya gracillima (Kuekenthal) that corresponds to a new GFP-like family named Akane

LUMINESCENCE
  • Yuko Kato
  • Mitsuru Jimbo
  • Youichi Sakakibara
  • Reiko Onizuka
  • Tatsuya Takahashi
  • Sachiko Matsuhashi
  • Hajime Mita
  • Kei Amada
  • Yukimitsu Imahara
  • Kimiko Tanabe
  • Akihisa Toda
  • Hisao Kamiya
  • 全て表示

32
6
開始ページ
1009
終了ページ
1016
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/bio.3284
出版者・発行元
WILEY

Certain marine organisms have been known to cause allergic reactions among occupational fishermen. We have previously reported that bronchial asthma among the workers engaged in spiny lobster fishing in Japan was caused by octocorals such as Dendronephthya sp. and Scleronephthya gracillima (previously named Alcyonium gracillimum).
Now we have found another octocoral, Scleronephthya gracillima (Kuekenthal), which causes the allergic disease in fishermen. The octocoral was characterized as a new green fluorescent protein (GFP)-like family. The new allergen has a molecular mass of 27 kDa in 1D and 2D SDS-PAGE under reduced conditions. The 27 kDa component was determined to be an allergen by western blotting, ECL immune staining method and absorption of patient sera with the antigen. Furthermore, the combination of analysis with LC-ESI-MS/MS and MASCOT search in the NCBInr database concluded the 27 kDa component had the sequence YPADI/LPDYFK, and that the 22 kDa component had the sequence QSFPEGFSWER, which both matched a GFP-like protein in Acropora aculeus and in Montastraea annularis. Further analysis by MALDI-TOF/MS/MS and MASCOT search in the NCBInr database of all 27 kDa eight spot components from 2D SDS-PAGE indicated that the sequence QSFPEGFSWER also matched as GFP-like protein in Lobophyllia hemprichii and Scleractinia sp. To our knowledge, this is the first report of the new allergenic protein that corresponds to a new GFP-like protein named Akane, and which has fluorescent emissions in the red and green part of the spectra at 628 nm and 508 nm, respectively.

リンク情報
DOI
https://doi.org/10.1002/bio.3284
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28378893
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000410296000015&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/bio.3284
  • ISSN : 1522-7235
  • eISSN : 1522-7243
  • PubMed ID : 28378893
  • Web of Science ID : WOS:000410296000015

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