1997
125I-iomazenil binding shows stress- and/or diazepam-induced reductions in mouse brain: Supporting data for 123I-iomazenil SPECT study of anxiety disorders
Annals of Nuclear Medicine
- ,
- ,
- ,
- Volume
- 11
- Number
- 3
- First page
- 243
- Last page
- 250
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1007/BF03164770
- Publisher
- Springer Tokyo
Effects of repeated swim stress on the binding of 125I-iomazenil were examined in the brains of diazepam-treated and non-treated mice. The mice were orally administered diazepam or vehicle (0.5% ethylene glycol) and subjected to daily swim stress (at 20°C for 10 min) for seven consecutive days. The distribution and the amount of 125I-iomazenil binding were analyzed autoradiographically after in vivo and in vitro binding experiments. Repeated swim stress decreased the in vivo binding in the hippocampus (p <
0.05) and cerebral cortex (p <
0.05) of vehicle-treated mice but caused no significant changes in diazepam-treated mice. Subchronic treatment with diazepam decreased the in vivo binding approximately 50% in all brain regions examined (p <
0.01). The in vitro experiment, however, revealed no significant changes except in the hippocampus, where a small but significant decrease in the binding was observed after subchronic treatment with diazepam (p <
0.01). The stress- or diazepam-induced reductions seem to represent alterations in the in vivo environment related to 125I-iomazenil binding. These results suggest that we can investigate the pathophysiology of stress and anxiety with 123I-iomazenil SPECT. Care must be taken concerning the effects of benzodiazepines.
0.05) and cerebral cortex (p <
0.05) of vehicle-treated mice but caused no significant changes in diazepam-treated mice. Subchronic treatment with diazepam decreased the in vivo binding approximately 50% in all brain regions examined (p <
0.01). The in vitro experiment, however, revealed no significant changes except in the hippocampus, where a small but significant decrease in the binding was observed after subchronic treatment with diazepam (p <
0.01). The stress- or diazepam-induced reductions seem to represent alterations in the in vivo environment related to 125I-iomazenil binding. These results suggest that we can investigate the pathophysiology of stress and anxiety with 123I-iomazenil SPECT. Care must be taken concerning the effects of benzodiazepines.
- Link information
- ID information
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- DOI : 10.1007/BF03164770
- ISSN : 0914-7187
- Pubmed ID : 9310174
- SCOPUS ID : 0030610217