酒井坦 (Hiroshi Sakai) - マイポータル - researchmap

基本情報

所属: 静岡県立大学大学院生活健康科学研究科教授

学位: 博士(農学)(東京大学)
農学修士(東京大学)

J-GLOBAL ID: 200901005418854812
researchmap会員ID: 1000207442

外部リンク: http://sfns.u-shizuoka-ken.ac.jp/proeng/

研究キーワード

2

研究分野

3

MISC

29

Novel glucoamylase-type enzymes from thermoactinoncyces vulgaris and Methanococcus janonaschii whose genes are found in the flanking region of the α-amylase gene

Appl. Microbiol. Biotechnol. 56(3/4) 465-473 2001年
Weakly bound calcium ions involved in the thevmostability of aqualysin I, a heat -stable sabtilisiu- type proteafe of ┣DBTherwus aquaricus(/)-┫DB YT-1

Biochiwica e⁺ Biopluqsica Acta 1433 132-138 1999年
新しい性質を獲得したアミラーゼ(共著)

バイオサイエンスとインダストリー 56(11) 37-38 1998年
Conversion of Nepoullulanase-alpha-amylase from Thermoactinomyces vulgaris R-47 to an Amylopullulanase-type Enzyme(Jointly Authored)

Journal of Biochemistry 123(2) 273-282 1998年
Manipulation of specificity of an α-amylase(Jointly authored)

56(11) 37-38 1998年
Conversion of Neopullulanase-alpha-amylase from Thermoactinomyces vulgaris R-47 into an Amylopullulanase-type Enzyme

Journal of Biochemistry 123(2) 275 1998年
An Aspergillus awamori acetylesterase: purification of the enzyme, and cloning and sequencing of the gene

T Koseki, S Furuse, K Iwano, H Sakai, H Matsuzawa

BIOCHEMICAL JOURNAL 326 485-490 1997年9月
4-alpha-glucanotransferase from the hyperthermophilic archaeon Thermococcus litoralis - Enzyme purification and characterization, and gene cloning, sequencing and expression in Escherichia coli

BS Jeon, H Taguchi, H Sakai, T Ohshima, T Wakagi, H Matsuzawa

EUROPEAN JOURNAL OF BIOCHEMISTRY 248(1) 171-178 1997年8月
4-alpha-Glucantransferase from the hyperthermophilic archaeon Thermococcus litoralis : Enzyme purification and characterization and gene cloning, sequencing and expression tn Escherichia coli(Jointly authored)

European Journal of Biochemistry 248(1) 171-178 1997年
An Aspergillus awamori acetylesterase : purification of the enzyme, and cloning and sequencing of the gene(Jointly authored)

Biochemical Journal 326(2) 485-490 1997年
Allosteric activation of L-lactate dehydrogenase analyzed by hybrid enzymes with effector-sensitive and -insensitive subunits

S Fushinobu, K Kamata, S Iwata, H Sakai, T Ohta, H Matsuzawa

JOURNAL OF BIOLOGICAL CHEMISTRY 271(41) 25611-25616 1996年10月
Allosteric Activation of L-Lactate Dehydrogenase Analyzed by Hybrid Enzymes with Effector-sensitive and-insensitive Subunits(Jointly authored)

Journal of Biological Chemistry 271(41) 25611-25616 1996年
Comparison of Transglycosylation Catalized by Two (]G1131[)-Amylases from Thermoactinomyces vulgaris R-47

Journal of Applied Glycoscience 43(1) 95-98 1996年
Molecular Cloning of the Genes for Pyruvate Kinase of Two Bacilli,Bacillus psychrophilus and Bacillus licheniformis,and Comparison of the Properties of the Enzymes Produced in Escherichia coli

Bioscience Biotechnology Biochemistry 59(8) 1536-1542 1995年
Comparison of primary structures and substrate specificities of two pullulan-hydrolyzing (]G1131[)-amylases,TVA (]G0001[) and TVA (]G0002[), from Thermoactinomyces vulgaris R-47

Biochimica et Biophysica Acta 1252(1) 35-42 1995年
Reddish Esherichia coli Cells Caused by Overproduction of Bacillus stearothermophilus Uroporpyrinogen (]G0003[)Methylase : Cloning, Sequencing, and Expression of the Gene

Pei-Lin Chou, Mari Ohtsuka, Takashi Minowa, Kazuhide Yamasat, Yoshiyuki Sakano, Hiroshi Matsuzawa, Takahisa Ohta, Hiroshi Sakai

Bioscience Biotechnology Biochemistry 59(10) 1817-1824 1995年
FUNCTIONAL ROLES OF TRP(337) AND GLU(632) IN CLOSTRIDIUM GLUCOAMYLASE, AS DETERMINED BY CHEMICAL MODIFICATION, MUTAGENESIS, AND THE STOPPED-FLOW METHOD

H OHNISHI, H MATSUMOTO, H SAKAI, T OHTA

JOURNAL OF BIOLOGICAL CHEMISTRY 269(5) 3503-3510 1994年2月
A convenient enzymic synthesis of 4²-(]J1131[)-isomaltosylisomaltose using Thermoactinomyces vulgaris R-47 Aalpha-amyase (]G0002[)(TVA(]G0002[))

Carbohydrate Research 261(1) 157-162 1994年
IDENTIFICATION OF GLUTAMIC ACID-204 AND ASPARTIC ACID-200 IN CHITINASE-A1 OF BACILLUS-CIRCULANS WL-12 AS ESSENTIAL RESIDUES FOR CHITINASE ACTIVITY

T WATANABE, K KOBORI, K MIYASHITA, T FUJII, H SAKAI, M UCHIDA, H TANAKA

JOURNAL OF BIOLOGICAL CHEMISTRY 268(25) 18567-18572 1993年9月
MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE GENE FOR PYRUVATE-KINASE OF BACILLUS-STEAROTHERMOPHILUS AND THE PRODUCTION OF THE ENZYME IN ESCHERICHIA-COLI - EVIDENCE THAT THE GENES FOR PHOSPHOFRUCTOKINASE AND PYRUVATE-KINASE CONSTITUTE AN OPERON

H SAKAI, T OHTA

EUROPEAN JOURNAL OF BIOCHEMISTRY 211(3) 851-859 1993年2月

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所属学協会

3

共同研究・競争的資金等の研究課題

8