論文

査読有り
2017年

Effect of Liposomes with Different Double Arms Polyethyleneglycol on Hepatic Metastasis Model Mice and Evaluation Using a Fluorescent Imaging Device

CURRENT DRUG DELIVERY
  • Ikumi Sugiyama
  • ,
  • Hiroki Oikawa
  • ,
  • Tomoyuki Masuda
  • ,
  • Yasuyuki Sadzuka

14
5
開始ページ
668
終了ページ
675
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.2174/1567201813666160328113653
出版者・発行元
BENTHAM SCIENCE PUBL LTD

Background: We have previously reported the synthesis of a novel polyethyleneglycol (PEG) lipid, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-PEG (DDA-PEG). This study aimed to clarify the anti-metastatic effect and localization of DDA-PEG-modified liposomes on a murine hepatic metastasis model.
Methods: M5076 ovarian sarcoma cells were inoculated for hepatic metastasis model mice. The accumulation of liposomes in the tumor and metastatic sites was detected by fluorescent imaging device. In metastasis study, doxorubicin (DOX) loaded DDA-PEG-modified liposome (DDA-LDOX) was injected. Alexa Fluor 790 NHS Ester loaded DDA-PEG-modified liposomes were used to detect fluorescence intensity at metastatic sites when visualized topically using a fluorescence imaging device.
Results: DDA-PEG-modified liposomes accumulated at the sites of hepatic metastasis but not in the normal hepatocytes. Furthermore, the DDA-LDOX inhibited metastasis in this model. The survival time of M5076 ovarian sarcoma bearing mice in DDA-LDOX group was longer than those in control, DOX solution and the other PEG-modified liposomal DOX groups, and the survival ratio in DDA-LDOX group remained 66.7% until 60 days after treatment.
Conclusion: It is expected that the DDA-PEG-modified liposomes will extensively contribute in clinical practice as a superior drug carrier because this liposomes proved to be effective against metastasis.


リンク情報
DOI
https://doi.org/10.2174/1567201813666160328113653
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000407013600008&DestApp=WOS_CPL

エクスポート
BibTeX RIS