論文

査読有り
2014年3月

A disintegrin and metalloproteinase 17 (ADAM17) mediates epidermal growth factor receptor transactivation by angiotensin II on hepatic stellate cells

LIFE SCIENCES
  • Hiroki Oikawa
  • ,
  • Chihaya Maesawa
  • ,
  • Yoshinori Tatemichi
  • ,
  • Yutaka Nishinari
  • ,
  • Masao Nishiya
  • ,
  • Hisata Mizugai
  • ,
  • Aya Ikeda
  • ,
  • Kanta Oikawa
  • ,
  • Yasuhiro Takikawa
  • ,
  • Tomoyuki Masuda

97
2
開始ページ
137
終了ページ
144
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.lfs.2013.12.028
出版者・発行元
PERGAMON-ELSEVIER SCIENCE LTD

Aims: Epidermal growth factor receptor (EGFR) transactivation induced by angiotensin II (Ang II) participates in the progression of various diseases. A disintegrin and metalloproteinase 17 (ADAM17) is thought to promote renal fibrosis, cardiac hypertrophy with fibrosis and atherosclerosis by activation of the EGFR through secretion of EGFR ligands. The purpose of this study was to investigate whether Ang II-induced EGFR transactivation occurs on hepatic stellate cells (HSCs) and whether the reaction is mediated via ADAM17.
Main methods: Ang ll-induced EGFR transactivation and cellular proliferation of the human HSC line LI90 were investigated using Western blotting and ATP assay, respectively. Ang ll-induced secretion of mature amphiregulin into the cell culture medium was evaluated by enzyme-linked immunosorbent assay (ELISA).
Key findings: An inhibitor of ADAM17, TAPI-1, as well as antagonists of EGFR and angiotensin II type-1 receptor (ATI), attenuated Ang ll-induced EGFR transactivation and proliferation of LI90 cells. Furthermore, silencing of ADAM17 inhibited Ang ll-induced secretion of mature amphiregulin in addition to EGFR transactivation.
Significance: These results indicate that ADAM17 mediates Ang II-induced EGFR transactivation on HSCs, and that this process may participate in the progression of liver fibrosis. (C) 2014 Elsevier Inc. All rights reserved.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.1016/j.lfs.2013.12.028
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000331920800007&DestApp=WOS_CPL

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